Experiment set1S382 for Bacteroides ovatus ATCC 8483
Pullulan 2.5 mg/mL carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + Pullulan (2.5 mg/mL)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 12 genes in this experiment
For carbon source Pullulan in Bacteroides ovatus ATCC 8483
For carbon source Pullulan across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Colanic acid biosynthesis | 1 |
| Iron acquisition in Vibrio | 1 |
| Ton and Tol transport systems | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Nicotinate and nicotinamide metabolism
- Fructose and mannose metabolism
- Ascorbate and aldarate metabolism
- Purine metabolism
- Pyrimidine metabolism
- Aminosugars metabolism
- Lipopolysaccharide biosynthesis
- Ether lipid metabolism
- Sphingolipid metabolism
- Thiamine metabolism
- Riboflavin metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- T cell receptor signaling pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NAD salvage pathway III (to nicotinamide riboside) | 3 | 2 | 1 |
| UTP and CTP dephosphorylation I | 7 | 5 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 7 | 3 |
| inosine 5'-phosphate degradation | 4 | 3 | 1 |
| guanosine nucleotides degradation III | 4 | 2 | 1 |
| adenosine nucleotides degradation I | 8 | 3 | 2 |
| purine nucleotides degradation I (plants) | 12 | 4 | 3 |
| guanosine nucleotides degradation II | 4 | 1 | 1 |
| guanosine nucleotides degradation I | 4 | 1 | 1 |
| adenosine nucleotides degradation II | 5 | 2 | 1 |
| superpathway of purines degradation in plants | 18 | 4 | 3 |
| superpathway of guanosine nucleotides degradation (plants) | 6 | 1 | 1 |
| ureide biosynthesis | 7 | 3 | 1 |
| tunicamycin biosynthesis | 9 | 1 | 1 |
| NAD salvage (plants) | 11 | 5 | 1 |