Experiment set1S372 for Escherichia coli HS(pFamp)R (ATCC 700891)
lim MOI1
Group: phageMedia: LB_plus_SM_buffer + Kan
Culturing: Ecoli_HSF_ML10, 48 well microplate; Epoch2, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Roniya on 3/13/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 2 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamate degradation II | 2 | 2 | 1 |
| L-aspartate degradation III (anaerobic) | 3 | 3 | 1 |
| L-aspartate degradation II (aerobic) | 3 | 3 | 1 |
| superpathway of L-aspartate and L-asparagine biosynthesis | 4 | 4 | 1 |
| L-canavanine degradation II | 6 | 1 | 1 |