Experiment set1S347 for Bacteroides ovatus ATCC 8483
RCM 1 x carbon source complex media
Group: carbon source complex mediaMedia: Varel_Bryant_medium + 1x RCM
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
RCM 1x includes: 10 g/L Bacto Peptone, 10 g/L Beef extract, 3 g/L Yeast Extract, 5 g/L D-Glucose, 5 g/L Sodium Chloride, 1 g/L Starch soluble, 0.5 g/L L-Cysteine hydrochloride monohydrate, 3 g/L Sodium acetate
Specific Phenotypes
For 5 genes in this experiment
For carbon source complex media RCM in Bacteroides ovatus ATCC 8483
For carbon source complex media RCM across organisms
Metabolic Maps
Color code by fitness: see overview map or list of maps.