Experiment set1S346 for Bacteroides ovatus ATCC 8483

MRS 1 x carbon source complex media

Group: carbon source complex media
Media: Varel_Bryant_medium + 1x MRS
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

MRS 1x includes: 56 mM D-Glucose, 10 g/L Proteose Peptone, 10 g/L Beef extract, 5 g/L Yeast Extract, 1 g/L Tween 80, 0.2 g/L Triammonium citrate, 0.5 g/L Sodium acetate, 0.2 g/L Potassium phosphate dibasic, 0.1 g/L Magnesium sulfate, 0.05 g/L Manganese (II) sulfate monohydrate, 8.25 mM L-Cysteine

Specific Phenotypes

For 4 genes in this experiment

For carbon source complex media MRS in Bacteroides ovatus ATCC 8483

For carbon source complex media MRS across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.