Experiment set1S345 for Bacteroides ovatus ATCC 8483
GAM 1 x carbon source complex media
Group: carbon source complex mediaMedia: Varel_Bryant_medium + 1x GAM
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
GAM 1x includes: 10 g/L Bacto Peptone, 3 g/L Soya peptone, 10 g/L Proteose Peptone, 13.5 g/L Digested serum, 5 g/L Yeast Extract, 2.2 g/L Meat extract, 1.2 g/L Liver extract, 3 g/L D-Glucose, 2.5 g/L Potassium phosphate monobasic, 3 g/L Sodium Chloride, 5 g/L Starch, 0.3 g/L L-Cysteine hydrochloride monohydrate, 0.3 g/L Sodium thioglycolate, 0.005 g/L Hemin, 0.001 g/L Menadione
Specific Phenotypes
For 12 genes in this experiment
For carbon source complex media GAM in Bacteroides ovatus ATCC 8483
For carbon source complex media GAM across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Campylobacter Iron Metabolism | 1 |
| De Novo Purine Biosynthesis | 1 |
| Multidrug Resistance Efflux Pumps | 1 |
| Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: