Experiment set1S333 for Acinetobacter radioresistens SK82

Ethanolamine nitrogen source

Group: nitrogen source
Media: RCH2_defined_noNitrogen + Ethanolamine (20 mM)
Culturing: Acinetobacter_SK82_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Kiani on 5/1/24
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 35 genes in this experiment

For nitrogen source Ethanolamine in Acinetobacter radioresistens SK82

For nitrogen source Ethanolamine across organisms

SEED Subsystems

Subsystem	#Specific
Phosphate metabolism	3
Cysteine Biosynthesis	2
Two cell division clusters relating to chromosome partitioning	2
Arginine and Ornithine Degradation	1
Copper homeostasis: copper tolerance	1
D-ribose utilization	1
Deoxyribose and Deoxynucleoside Catabolism	1
Glutathione: Non-redox reactions	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Lactate utilization	1
Methylglyoxal Metabolism	1
Proteasome bacterial	1
Proteolysis in bacteria, ATP-dependent	1
Respiratory dehydrogenases 1	1
Ribonucleotide reduction	1
Universal GTPases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyruvate metabolism
• Pentose phosphate pathway
• Urea cycle and metabolism of amino groups
• Pyrimidine metabolism
• Glutamate metabolism
• Geraniol degradation
• Lysine biosynthesis
• Arginine and proline metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Glutathione metabolism
• Lipopolysaccharide biosynthesis
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• Butanoate metabolism
• Nicotinate and nicotinamide metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450
• Biosynthesis of unsaturated fatty acids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyruvate fermentation to (R)-lactate	1	1	1
adenosylcobalamin salvage from cobalamin	5	5	4
adenosylcobinamide-GDP salvage from cobinamide I	5	5	3
4-aminobutanoate degradation III	2	2	1
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I	2	2	1
L-lactaldehyde degradation (aerobic)	2	2	1
adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide	6	4	3
adenosylcobinamide-GDP salvage from cobinamide II	6	4	3
cobalamin salvage (eukaryotic)	8	4	4
D-lactate to cytochrome bo oxidase electron transfer	2	1	1
oleate biosynthesis II (animals and fungi)	2	1	1
palmitoleate biosynthesis IV (fungi and animals)	2	1	1
ribose phosphorylation	2	1	1
homotaurine degradation	2	1	1
superpathway of adenosylcobalamin salvage from cobinamide I	8	8	3
L-alanine degradation II (to D-lactate)	3	3	1
superpathway of adenosylcobalamin salvage from cobinamide II	9	7	3
methylglyoxal degradation V	3	2	1
methylglyoxal degradation IV	3	2	1
2-deoxy-D-ribose degradation I	3	2	1
molybdenum cofactor biosynthesis	3	1	1
vancomycin resistance I	3	1	1
2-chloroacrylate degradation I	3	1	1
4-hydroxy-2-nonenal detoxification	4	1	1
lactate biosynthesis (archaea)	5	2	1
pentachlorophenol degradation	10	3	2
L-alanine degradation VI (reductive Stickland reaction)	6	2	1
pyrimidine deoxyribonucleotides de novo biosynthesis II	7	5	1
superpathway of thiamine diphosphate biosynthesis III (eukaryotes)	7	3	1
superpathway of methylglyoxal degradation	8	5	1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales)	8	4	1
glutathione-mediated detoxification I	8	3	1
L-rhamnose degradation II	8	2	1
sorgoleone biosynthesis	8	2	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	6	1
superpathway of L-alanine fermentation (Stickland reaction)	9	4	1
glutathione-mediated detoxification II	9	1	1
gliotoxin biosynthesis	9	1	1
superpathway of thiamine diphosphate biosynthesis I	10	6	1
superpathway of thiamine diphosphate biosynthesis II	11	8	1
adenosylcobalamin biosynthesis II (aerobic)	33	17	3
adenosylcobalamin biosynthesis I (anaerobic)	36	16	3
superpathway of fucose and rhamnose degradation	12	4	1
indole glucosinolate activation (intact plant cell)	12	3	1
camalexin biosynthesis	12	2	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)	14	12	1
mixed acid fermentation	16	12	1
superpathway of glucose and xylose degradation	17	10	1
heterolactic fermentation	18	9	1