Experiment set1S331 for Acinetobacter radioresistens SK82
casamino acids nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_noNitrogen + casamino acids (5 mg/mL)
Culturing: Acinetobacter_SK82_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Kiani on 5/1/24
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 5 genes in this experiment
For nitrogen source casamino acids in Acinetobacter radioresistens SK82
For nitrogen source casamino acids across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Periplasmic disulfide interchange | 1 |
| Proteolysis in bacteria, ATP-dependent | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| assimilatory sulfate reduction III | 3 | 3 | 1 |
| assimilatory sulfate reduction I | 4 | 3 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 8 | 1 |
| superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) | 10 | 6 | 1 |
| superpathway of L-methionine biosynthesis (by sulfhydrylation) | 12 | 11 | 1 |