Experiment set1S321 for Bacteroides ovatus ATCC 8483

B-glucan from yeast 2.5 mg/mL carbon source

Group: carbon source
Media: Varel_Bryant_medium + B-glucan from yeast (2.5 mg/mL)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 8 genes in this experiment

For carbon source B-glucan from yeast in Bacteroides ovatus ATCC 8483

For carbon source B-glucan from yeast across organisms

SEED Subsystems

Subsystem	#Specific
Beta-Glucoside Metabolism	1
Fructooligosaccharides(FOS) and Raffinose Utilization	1
Glycogen metabolism	1
Maltose and Maltodextrin Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Starch and sucrose metabolism
• Cyanoamino acid metabolism
• Other glycan degradation
• Sphingolipid metabolism
• Phenylpropanoid biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
neolinustatin bioactivation	3	2	2
linustatin bioactivation	4	2	2
lotaustralin degradation	2	1	1
linamarin degradation	2	1	1
glycogen degradation I	8	7	3
glycogen degradation II	6	5	2
cellulose degradation II (fungi)	3	2	1
starch degradation V	4	3	1
starch degradation III	4	2	1
coumarin biosynthesis (via 2-coumarate)	5	2	1
α-tomatine degradation	6	1	1
sucrose biosynthesis II	8	5	1
firefly bioluminescence	14	2	1