Experiment set1S32 for Rhodanobacter sp. FW510-T8
R2A_PIPES with Metal_ion_mix_sitestressplate 0.5 x
Group: stressMedia: R2A_PIPES + 0.5x Metal_ion_mix_sitestressplate, pH=7
Culturing: rhodanobacter_T8_ML1, 96 well deep well block, Aerobic, at 23 (C), shaken=700 rpm
Growth: about 4.6 generations
By: Hans and Hira on 1/10/25
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate, 30 mM PIPES sesquisodium salt
Metal_ion_mix_sitestressplate 0.5x includes: 0.004 mM Cobalt chloride hexahydrate, 0.001 mM Cadmium chloride hemipentahydrate, 0.0265 mM Nickel (II) chloride hexahydrate, 0.625 mM Aluminum chloride hydrate, 0.5 mM Manganese (II) chloride tetrahydrate, 0.005 mM Zinc chloride (final concentrations)
Specific Phenotypes
None in this experiment