Experiment set1S310 for Bacteroides ovatus ATCC 8483

Dextran, Mw ~200,000 2.5 mg/mL carbon source

Group: carbon source
Media: Varel_Bryant_medium + dextran, Mw ~200,000 (2.5 mg/mL)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 9 genes in this experiment

For carbon source dextran, Mw ~200,000 in Bacteroides ovatus ATCC 8483

For carbon source dextran, Mw ~200,000 across organisms

SEED Subsystems

Subsystem	#Specific
LOS core oligosaccharide biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Starch and sucrose metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
starch degradation I	3	1	1
glycogen degradation I	8	7	1