Experiment set1S305 for Acinetobacter radioresistens SK82

L-Glutamine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Glutamine (20 mM)
Culturing: Acinetobacter_SK82_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Kiani on 5/1/24
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 23 genes in this experiment

For carbon source L-Glutamine in Acinetobacter radioresistens SK82

For carbon source L-Glutamine across organisms

SEED Subsystems

Subsystem	#Specific
Heat shock dnaK gene cluster extended	2
Methionine Biosynthesis	2
Campylobacter Iron Metabolism	1
Glutaredoxins	1
Glutathione: Redox cycle	1
High affinity phosphate transporter and control of PHO regulon	1
One-carbon metabolism by tetrahydropterines	1
Phosphate metabolism	1
Rhamnose containing glycans	1
Serine-glyoxylate cycle	1
ZZ gjo need homes	1
dTDP-rhamnose synthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• One carbon pool by folate
• Fatty acid biosynthesis
• Methionine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• alpha-Linolenic acid metabolism
• Naphthalene and anthracene degradation
• Benzoate degradation via CoA ligation
• Methane metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-methionine salvage from L-homocysteine	3	1	1
L-methionine biosynthesis III	4	4	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
L-methionine biosynthesis I	5	3	1
folate transformations I	13	8	2
superpathway of L-homoserine and L-methionine biosynthesis	8	6	1
folate transformations III (E. coli)	9	8	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	7	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	7	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
folate transformations II (plants)	11	9	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	11	1
superpathway of L-methionine salvage and degradation	16	5	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
aspartate superpathway	25	22	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1