Experiment set1S304 for Bacteroides ovatus ATCC 8483

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Inulin from chicory 2.5 mg/mL carbon source

Group: carbon source
Media: Varel_Bryant_medium + Inulin from chicory (2.5 mg/mL)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 6 genes in this experiment

For carbon source Inulin from chicory in Bacteroides ovatus ATCC 8483

For carbon source Inulin from chicory across organisms

SEED Subsystems

Subsystem #Specific
Fructose utilization 1
Mannitol Utilization 1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization 1
Sucrose utilization 1
Sucrose utilization Shewanella 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-sorbitol degradation I 3 2 1
sucrose degradation I (sucrose phosphotransferase) 3 1 1
sucrose degradation IV (sucrose phosphorylase) 4 3 1
sucrose degradation III (sucrose invertase) 4 3 1
sucrose degradation VII (sucrose 3-dehydrogenase) 4 1 1
mannitol cycle 5 3 1
sucrose degradation II (sucrose synthase) 5 3 1
heterolactic fermentation 18 14 1
superpathway of anaerobic sucrose degradation 19 14 1