Experiment set1S130 for Escherichia coli HS(pFamp)R (ATCC 700891)
BWC1S MOI1
Group: phageMedia: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: Ecoli_HSF_ML10, 48 well microplate; Epoch2, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Roniya on 2/14/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 1 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| NAD and NADP cofactor biosynthesis global | 1 |
| NAD regulation | 1 |
| Redox-dependent regulation of nucleus processes | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Nicotinate and nicotinamide metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NAD salvage pathway V (PNC V cycle) | 5 | 3 | 1 |
| NAD salvage pathway I (PNC VI cycle) | 7 | 5 | 1 |
| NAD salvage (plants) | 11 | 5 | 1 |
| superpathway of NAD biosynthesis in eukaryotes | 14 | 7 | 1 |