Experiment set1S124 for Escherichia coli ECOR27
TP2
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 11/14/2024
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 3 genes in this experiment
For phage MOI 10 in Escherichia coli ECOR27
For phage MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| DNA repair, UvrABC system | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Ascorbate and aldarate metabolism
- Ubiquinone and menaquinone biosynthesis
- Nucleotide sugars metabolism
- Glycosaminoglycan degradation
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| dTDP-N-acetylthomosamine biosynthesis | 4 | 4 | 1 |
| superpathway of enterobacterial common antigen biosynthesis | 10 | 9 | 1 |
| peptidoglycan recycling II | 10 | 7 | 1 |
| peptidoglycan recycling I | 14 | 14 | 1 |
| superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis | 19 | 5 | 1 |