Experiment set1S112 for Escherichia coli ECOR27
Bas69
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: Ecoli_ECOR27_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 11/14/2024
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 11 genes in this experiment
For phage MOI 10 in Escherichia coli ECOR27
For phage MOI 10 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| KDO2-Lipid A biosynthesis | 1 |
| Oxidative stress | 1 |
| Ribosomal protein S12p Asp methylthiotransferase | 1 |
| Transport of Zinc | 1 |
| tRNA processing | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Lipopolysaccharide biosynthesis
- Fructose and mannose metabolism
- Puromycin biosynthesis
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- High-mannose type N-glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Keratan sulfate biosynthesis
- Peptidoglycan biosynthesis
- Glycerolipid metabolism
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- Sphingolipid metabolism
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - ganglio series
- Carotenoid biosynthesis - General
- Zeatin biosynthesis
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Flavone and flavonol biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| enterobacterial common antigen biosynthesis | 5 | 4 | 2 |
| superpathway of enterobacterial common antigen biosynthesis | 10 | 9 | 3 |
| dTDP-N-acetylthomosamine biosynthesis | 4 | 4 | 1 |
| superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis | 19 | 5 | 1 |