Experiment set1IT094 for Escherichia coli BW25113

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casamino acids nitrogen source

200 most detrimental genes:

  gene name fitness t score description  
b1101 ptsG +3.0 25.7 fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI) compare
b2741 rpoS +1.8 13.3 RNA polymerase sigma factor (NCBI) compare
b1361 ydaW +1.0 1.9 orf, hypothetical protein (VIMSS) compare
b4402 yjjY +1.0 2.1 hypothetical protein (NCBI) compare
b1103 ycfF +0.9 2.4 orf, hypothetical protein (VIMSS) compare
b1085 b1085 +0.9 2.5 orf, hypothetical protein (VIMSS) compare
b2377 yfdY +0.9 3.1 predicted inner membrane protein (NCBI) compare
b0866 ybjQ +0.9 1.7 hypothetical protein (NCBI) compare
b2229 b2229 +0.8 1.8 orf, hypothetical protein (VIMSS) compare
b0014 dnaK +0.8 2.1 molecular chaperone DnaK (NCBI) compare
b2012 yeeD +0.8 3.1 hypothetical protein (NCBI) compare
b4505 insX +0.8 1.7 predicted IS protein (RefSeq) compare
b2051 wcaH +0.7 1.5 GDP-mannose mannosyl hydrolase (VIMSS) compare
b0782 moaB +0.7 2.0 molybdopterin biosynthesis protein B (NCBI) compare
b0998 torD +0.7 1.8 chaperone protein TorD (NCBI) compare
b1518 lsrG +0.7 2.4 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b3261 fis +0.7 2.6 DNA-binding protein Fis (NCBI) compare
b1842 holE +0.6 1.7 DNA polymerase III, theta subunit (NCBI) compare
b0912 ihfB +0.6 2.6 integration host factor subunit beta (NCBI) compare
b1648 b1648 +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b4653 yicT +0.6 2.1 no description compare
b1749 xthA +0.6 2.9 exonuclease III (NCBI) compare
b1777 b1777 +0.6 1.7 orf, hypothetical protein (VIMSS) compare
b1681 sufD +0.6 4.2 component of SufBCD complex (NCBI) compare
b1400 paaY +0.6 1.5 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b4416 rybA +0.6 1.4 unknown RNA (NCBI) compare
b1683 ynhE +0.6 4.1 orf, hypothetical protein (VIMSS) compare
b4603 rseX +0.6 1.1 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1104 ycfL +0.6 1.7 hypothetical protein (NCBI) compare
b0059 hepA +0.5 4.8 ATP-dependent helicase HepA (NCBI) compare
b4158 yjeO +0.5 2.3 conserved inner membrane protein (NCBI) compare
b0641 rlpB +0.5 1.7 minor lipoprotein (NCBI) compare
b2287 nuoB +0.5 2.1 NADH dehydrogenase subunit B (NCBI) compare
b2990 hybG +0.5 1.2 hydrogenase 2 accessory protein (NCBI) compare
b3672 ivbL +0.5 1.4 ilvB operon leader peptide (NCBI) compare
b1961 dcm +0.5 1.4 DNA cytosine methylase (NCBI) compare
b1368 ynaA +0.5 2.5 Rac prophage; predicted tail protein (RefSeq) compare
b1465 narV +0.5 1.6 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b1758 b1758 +0.5 1.1 putative cytochrome oxidase (VIMSS) compare
b1824 yobF +0.5 2.5 hypothetical protein (NCBI) compare
b4410 ecnA +0.5 1.0 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b2673 nrdH +0.5 1.7 glutaredoxin-like protein (NCBI) compare
b1712 ihfA +0.5 1.8 integration host factor subunit alpha (NCBI) compare
b1591 b1591 +0.5 1.1 putative oxidoreductase component (VIMSS) compare
b2498 upp +0.5 2.1 uracil phosphoribosyltransferase (VIMSS) compare
b1729 ydjN +0.5 1.9 predicted transporter (NCBI) compare
b3198 kdsC +0.5 1.9 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b3643 rph +0.5 1.9 ribonuclease PH (NCBI) compare
b2291 yfbR +0.5 1.4 hypothetical protein (NCBI) compare
b0661 miaB +0.5 3.4 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b0825 mipB +0.5 2.0 putative transaldolase (VIMSS) compare
b2684 mprA +0.5 1.8 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0798 ybiA +0.5 1.3 hypothetical protein (NCBI) compare
b2285 nuoE +0.5 1.3 NADH dehydrogenase subunit E (NCBI) compare
b1569 dicC +0.5 1.6 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b2201 ccmA +0.5 2.3 ATP binding protein of heme exporter A (VIMSS) compare
b0784 moaD +0.5 1.0 molybdopterin synthase, small subunit (NCBI) compare
b1074 flgC +0.4 1.0 flagellar basal-body rod protein C (NCBI) compare
b1682 sufC +0.4 2.5 cysteine desulfurase ATPase component (NCBI) compare
b4511 ybdZ +0.4 1.7 hypothetical protein (NCBI) compare
b1357 ydaS +0.4 0.9 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1789 yeaL +0.4 1.5 conserved inner membrane protein (NCBI) compare
b4458 oxyS +0.4 0.7 global regulatory RNA (NCBI) compare
b1999 yeeP +0.4 2.0 putative histone (VIMSS) compare
b0843 ybjH +0.4 0.8 hypothetical protein (NCBI) compare
b3863 polA +0.4 0.7 DNA polymerase I (NCBI) compare
b0388 aroL +0.4 2.3 shikimate kinase II (NCBI) compare
b0909 ycaL +0.4 2.4 putative heat shock protein (VIMSS) compare
b4363 yjjB +0.4 1.4 conserved inner membrane protein (RefSeq) compare
b1446 ydcY +0.4 1.1 hypothetical protein (NCBI) compare
b2421 cysM +0.4 1.8 cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI) compare
b3190 yrbA +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b1679 sufE +0.4 1.9 cysteine desufuration protein SufE (NCBI) compare
b1680 sufS +0.4 2.5 selenocysteine lyase (NCBI) compare
b1524 yneH +0.4 0.9 predicted glutaminase (NCBI) compare
b3149 yraO +0.4 2.5 DnaA initiator-associating factor for replication initiation (NCBI) compare
b1557 cspB +0.4 0.8 Qin prophage; cold shock protein (NCBI) compare
b0706 ybfD +0.4 2.0 hypothetical protein (NCBI) compare
b2419 yfeK +0.4 1.2 hypothetical protein (NCBI) compare
b1231 tyrT +0.4 0.7 tRNA-Tyr (NCBI) compare
b4391 yjjK +0.4 2.9 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0157 yadS +0.4 1.8 conserved inner membrane protein (NCBI) compare
b4687 shoB +0.4 2.0 toxic membrane protein (RefSeq) compare
b0865 ybjP +0.4 1.7 predicted lipoprotein (NCBI) compare
b4451 ryhB +0.4 0.8 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b3686 ibpB +0.4 2.1 heat shock chaperone (NCBI) compare
b1672 ydhW +0.4 1.4 hypothetical protein (NCBI) compare
b2398 yfeC +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b0788 ybhN +0.4 1.6 conserved inner membrane protein (NCBI) compare
b2435 amiA +0.4 1.8 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b1841 yobA +0.4 0.6 hypothetical protein (NCBI) compare
b0423 thiI +0.4 2.2 thiamine biosynthesis protein ThiI (NCBI) compare
b2743 pcm +0.4 1.3 protein-L-isoaspartate O-methyltransferase (NCBI) compare
b2846 yqeH +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b2314 dedD +0.4 1.6 putative lipoprotein (VIMSS) compare
b1390 paaC +0.4 1.6 predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI) compare
b2128 yehW +0.4 1.9 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0736 ybgC +0.4 1.3 predicted acyl-CoA thioesterase (NCBI) compare
b1095 fabF +0.4 2.6 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b0735 ybgE +0.4 1.4 conserved inner membrane protein (NCBI) compare
b3106 yhaK +0.4 1.5 predicted pirin-related protein (NCBI) compare
b1259 yciG +0.4 0.8 orf, hypothetical protein (VIMSS) compare
b1628 rsxB +0.4 1.3 electron transport complex protein RnfB (NCBI) compare
b0250 ykfB +0.4 1.8 CP4-6 prophage; predicted protein (NCBI) compare
b4097 phnK +0.4 1.3 carbon-phosphorus lyase complex subunit (NCBI) compare
b1731 cedA +0.4 1.5 cell division modulator (RefSeq) compare
b4578 insP +0.4 1.1 no description compare
b4085 alsE +0.4 1.9 allulose-6-phosphate 3-epimerase (NCBI) compare
b4176 yjeT +0.4 1.2 conserved inner membrane protein (NCBI) compare
b2602 yfiL +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b2786 barA +0.4 3.3 hybrid sensory histidine kinase, in two-component regulatory system with UvrY (NCBI) compare
b2129 yehX +0.4 2.1 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b0746 valZ +0.3 1.1 tRNA-Val (NCBI) compare
b1415 aldA +0.3 1.9 Lactaldehyde / glycolaldehyde dehydrogenase aldA (EC 1.2.1.22; EC 1.2.1.21) (from data) compare
b4369 leuP +0.3 0.9 tRNA-Leu (NCBI) compare
b4096 phnL +0.3 1.4 carbon-phosphorus lyase complex subunit (NCBI) compare
b0426 yajQ +0.3 1.8 orf, hypothetical protein (VIMSS) compare
b2057 wcaC +0.3 2.3 predicted glycosyl transferase (NCBI) compare
b2455 eutE +0.3 1.7 predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI) compare
b1528 ydeA +0.3 2.1 sugar efflux transporter (NCBI) compare
b2688 gshA +0.3 2.2 glutamate--cysteine ligase (NCBI) compare
b0990 cspG +0.3 1.1 DNA-binding transcriptional regulator (NCBI) compare
b2123 yehR +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b3676 yidH +0.3 1.5 conserved inner membrane protein (NCBI) compare
b1355 b1355 +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b4490 efeU +0.3 2.3 no description compare
b3221 yhcH +0.3 1.8 hypothetical protein (NCBI) compare
b0405 queA +0.3 1.6 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI) compare
b1384 feaR +0.3 2.0 DNA-binding transcriptional dual regulator (NCBI) compare
b2195 ccmG +0.3 0.8 periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI) compare
b0686 ybfF +0.3 1.6 hypothetical protein (NCBI) compare
b2582 trxC +0.3 1.3 thioredoxin 2 (NCBI) compare
b2597 yfiA +0.3 0.8 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b3356 yhfA +0.3 1.6 hypothetical protein (NCBI) compare
b1656 sodB +0.3 0.9 superoxide dismutase, Fe (NCBI) compare
b1531 marA +0.3 1.3 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b3675 yidG +0.3 2.0 predicted inner membrane protein (NCBI) compare
b0407 yajC +0.3 1.2 preprotein translocase subunit YajC (NCBI) compare
b1523 yneG +0.3 0.9 hypothetical protein (NCBI) compare
b3181 greA +0.3 1.7 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b4411 ecnB +0.3 1.2 entericidin B membrane lipoprotein (NCBI) compare
b1037 csgG +0.3 2.2 outer membrane lipoprotein (NCBI) compare
b3029 ygiN +0.3 1.0 quinol monooxygenase (NCBI) compare
b1914 uvrY +0.3 1.9 response regulator (NCBI) compare
b0615 citF +0.3 1.3 citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI) compare
b0015 dnaJ +0.3 2.2 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b1421 trg +0.3 1.5 methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI) compare
b4071 nrfB +0.3 1.0 formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS) compare
b1823 cspC +0.3 1.0 stress protein, member of the CspA-family (NCBI) compare
b0624 crcB +0.3 1.2 camphor resistance protein CrcB (NCBI) compare
b3293 yhdN +0.3 1.6 hypothetical protein (NCBI) compare
b1143 ymfI +0.3 0.5 orf, hypothetical protein (VIMSS) compare
b3243 aaeR +0.3 1.5 predicted DNA-binding transcriptional regulator, efflux system (NCBI) compare
b4327 yjiE +0.3 1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b0503 ybbB +0.3 0.9 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b2468 aegA +0.3 2.1 fused predicted oxidoreductase: FeS binding subunit/NAD/FAD-binding subunit (NCBI) compare
b2267 elaA +0.3 1.4 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b1480 sra +0.3 1.0 30S ribosomal subunit protein S22 (NCBI) compare
b1960 vsr +0.3 1.1 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b4609 ryfD +0.3 1.3 Novel sRNA, function unknown (RefSeq) compare
b0218 yafU +0.3 1.4 predicted inner membrane protein (RefSeq) compare
b4478 dgoD +0.3 2.0 galactonate dehydratase (NCBI) compare
b1070 flgN +0.3 0.5 export chaperone for FlgK and FlgL (NCBI) compare
b0013 yaaI +0.3 1.6 hypothetical protein (NCBI) compare
b0225 yafQ +0.3 1.2 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b2259 pmrD +0.3 0.7 polymyxin resistance protein B (VIMSS) compare
b2390 ypeC +0.3 1.2 hypothetical protein (NCBI) compare
b0806 ybiM +0.3 1.0 orf, hypothetical protein (VIMSS) compare
b4544 yfbW +0.3 1.4 hypothetical protein (NCBI) compare
b1959 yedA +0.3 1.2 predicted inner membrane protein (NCBI) compare
b2725 hycA +0.3 1.5 regulator of the transcriptional regulator FhlA (NCBI) compare
b2918 argK +0.3 1.4 arginine/ornithine transport system ATPase (NCBI) compare
b2562 yfhL +0.3 2.1 predicted 4Fe-4S cluster-containing protein (NCBI) compare
b3658 selC +0.3 0.9 tRNA-Sec (NCBI) compare
b2587 kgtP +0.3 2.0 alpha-ketoglutarate transporter (NCBI) compare
b2736 ygbJ +0.3 1.5 predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI) compare
b3095 yqjA +0.3 2.2 conserved inner membrane protein (NCBI) compare
b4293 fecI +0.3 1.0 KpLE2 phage-like element; RNA polymerase, sigma 19 factor (NCBI) compare
b0560 nohB +0.3 0.8 DLP12 prophage; DNA packaging protein (NCBI) compare
b2954 yggV +0.3 0.9 putative deoxyribonucleotide triphosphate pyrophosphatase (NCBI) compare
b2956 yggM +0.3 1.8 hypothetical protein (NCBI) compare
b2724 hycB +0.3 1.6 hydrogenase 3, Fe-S subunit (NCBI) compare
b3857 mobA +0.3 1.9 molybdopterin-guanine dinucleotide biosynthesis protein A (NCBI) compare
b0830 yliB +0.3 2.2 predicted peptide transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1427 rimL +0.3 1.1 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b1811 yoaH +0.3 1.1 hypothetical protein (NCBI) compare
b3166 truB +0.3 1.9 tRNA pseudouridine synthase B (NCBI) compare
b0487 cueR +0.3 1.2 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b2706 gutM +0.3 0.9 DNA-binding transcriptional activator of glucitol operon (NCBI) compare
b2732 ygbA +0.3 1.6 hypothetical protein (NCBI) compare
b3213 gltD +0.3 2.2 glutamate synthase, 4Fe-4S protein, small subunit (NCBI) compare
b0535 fimZ +0.3 2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2222 atoA +0.3 1.6 acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (NCBI) compare
b1613 manA +0.3 0.9 mannose-6-phosphate isomerase (VIMSS) compare
b2410 yfeH +0.3 1.3 predicted inner membrane protein (NCBI) compare
b2922 yggE +0.3 2.0 hypothetical protein (NCBI) compare
b4002 yjaI +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b0468 ybaN +0.3 1.3 conserved inner membrane protein (NCBI) compare
b0946 ycbW +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b0213 yafS +0.3 1.6 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

For 5 genes in this experiment

For nitrogen source casamino acids in Escherichia coli BW25113

For nitrogen source casamino acids across organisms