Experiment set1IT093 for Escherichia coli BW25113

Compare to:

casamino acids nitrogen source

200 most important genes:

  gene name fitness t score description  
b2476 purC -7.0 -4.8 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b1261 trpB -6.6 -4.5 tryptophan synthase subunit beta (NCBI) compare
b1260 trpA -6.4 -6.2 tryptophan synthase subunit alpha (NCBI) compare
b0523 purE -5.8 -4.0 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b4245 pyrB -5.7 -10.2 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b2312 purF -5.6 -6.6 amidophosphoribosyltransferase (NCBI) compare
b4006 purH -5.3 -9.6 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b0033 carB -5.2 -13.6 carbamoyl-phosphate synthase large subunit (NCBI) compare
b2499 purM -5.1 -5.0 phosphoribosylaminoimidazole synthetase (NCBI) compare
b1062 pyrC -5.1 -7.7 dihydroorotase (NCBI) compare
b0032 carA -5.0 -8.3 carbamoyl-phosphate synthase small subunit (NCBI) compare
b0907 serC -4.9 -8.8 phosphoserine aminotransferase (NCBI) compare
b0945 pyrD -4.8 -11.6 dihydroorotate dehydrogenase (NCBI) compare
b4005 purD -4.8 -8.0 phosphoribosylamine--glycine ligase (NCBI) compare
b1281 pyrF -4.8 -4.6 orotidine 5'-phosphate decarboxylase (NCBI) compare
b0522 purK -4.5 -9.1 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b3642 pyrE -4.5 -4.4 orotate phosphoribosyltransferase (NCBI) compare
b2557 purL -4.3 -10.4 phosphoribosylformylglycinamidine synthase (NCBI) compare
b2320 pdxB -4.3 -10.0 erythronate-4-phosphate dehydrogenase (NCBI) compare
b1263 trpD -4.3 -4.1 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b0052 pdxA -4.2 -10.2 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI) compare
b2500 purN -4.2 -5.2 phosphoribosylglycinamide formyltransferase (NCBI) compare
b2564 pdxJ -4.2 -4.9 pyridoxal phosphate biosynthetic protein (NCBI) compare
b0142 folK -4.0 -2.6 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1262 trpC -3.7 -8.2 N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS) compare
b0145 dksA -3.5 -4.8 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0437 clpP -3.4 -9.6 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1291 sapD -3.3 -3.2 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2501 ppk -3.3 -12.4 polyphosphate kinase (NCBI) compare
b3463 ftsE -3.2 -4.4 predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b2329 aroC -3.2 -7.3 chorismate synthase (NCBI) compare
b3082 ygjM -3.1 -4.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b3389 aroB -3.0 -12.2 3-dehydroquinate synthase (NCBI) compare
b1264 trpE -3.0 -7.0 anthranilate synthase component I (NCBI) compare
b0049 apaH -2.9 -6.9 diadenosinetetraphosphatase (NCBI) compare
b3281 aroE -2.8 -16.3 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3613 yibP -2.7 -5.7 putative membrane protein (VIMSS) compare
b2188 yejM -2.6 -9.8 predicted hydrolase, inner membrane (NCBI) compare
b0908 aroA -2.6 -10.8 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3405 ompR -2.5 -5.6 osmolarity response regulator (NCBI) compare
b3870 glnA -2.5 -2.4 glutamine synthetase (NCBI) compare
b4451 ryhB -2.5 -2.4 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b0738 tolR -2.5 -4.0 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b4177 purA -2.4 -1.7 adenylosuccinate synthetase (NCBI) compare
b4246 pyrL -2.4 -1.4 pyrBI operon leader peptide (NCBI) compare
b0737 tolQ -2.3 -4.6 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1545 pinQ -2.3 -2.5 Qin prophage; predicted site-specific recombinase (NCBI) compare
b3390 aroK -2.3 -3.4 shikimate kinase I (NCBI) compare
b0740 tolB -2.2 -6.1 translocation protein TolB precursor (NCBI) compare
b0118 acnB -2.2 -3.9 aconitate hydratase (NCBI) compare
b2830 nudH -2.2 -2.5 dinucleoside polyphosphate hydrolase (NCBI) compare
b0750 nadA -2.1 -5.0 quinolinate synthetase (NCBI) compare
b2318 truA -2.1 -5.2 tRNA pseudouridine synthase A (NCBI) compare
b1130 phoP -2.1 -7.2 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b1693 aroD -2.1 -7.1 3-dehydroquinate dehydratase (NCBI) compare
b1677 lpp -2.1 -3.8 murein lipoprotein (NCBI) compare
b4388 serB -2.1 -7.0 3-phosphoserine phosphatase (NCBI) compare
b3725 pstB -2.0 -2.4 phosphate transporter subunit (NCBI) compare
b3811 xerC -2.0 -3.3 site-specific tyrosine recombinase XerC (NCBI) compare
b4172 hfq -2.0 -1.9 RNA-binding protein Hfq (NCBI) compare
b1129 phoQ -2.0 -8.2 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b0134 panB -2.0 -7.7 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI) compare
b3462 ftsX -2.0 -6.2 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0438 clpX -2.0 -8.2 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0114 aceE -1.9 -3.4 pyruvate dehydrogenase subunit E1 (NCBI) compare
b0890 ftsK -1.9 -7.7 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0741 pal -1.9 -4.9 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b2313 cvpA -1.8 -2.7 membrane protein required for colicin V production (NCBI) compare
b0109 nadC -1.8 -6.5 nicotinate-nucleotide pyrophosphorylase (NCBI) compare
b3728 pstS -1.8 -5.4 phosphate transporter subunit (NCBI) compare
b2820 recB -1.8 -2.4 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b3250 mreC -1.8 -2.6 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b0739 tolA -1.8 -4.7 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3121 yhaC -1.7 -2.3 hypothetical protein (NCBI) compare
b3741 gidA -1.7 -7.7 glucose-inhibited division protein A (NCBI) compare
b3764 yifE -1.7 -5.4 hypothetical protein (NCBI) compare
b3459 yhhK -1.7 -6.0 hypothetical protein (NCBI) compare
b0775 bioB -1.6 -4.9 biotin synthase (NCBI) compare
b2567 rnc -1.6 -1.8 ribonuclease III (NCBI) compare
b0133 panC -1.6 -6.6 pantoate--beta-alanine ligase (NCBI) compare
b1480 sra -1.6 -2.9 30S ribosomal subunit protein S22 (NCBI) compare
b4443 gcvB -1.6 -2.1 GcvB sRNA gene divergent from gcvA (RefSeq) compare
b2913 serA -1.6 -7.5 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b0630 lipB -1.5 -2.2 protein of lipoate biosynthesis (VIMSS) compare
b1292 sapC -1.5 -2.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1138 ymfE -1.5 -2.2 e14 prophage; predicted inner membrane protein (NCBI) compare
b1850 eda -1.5 -2.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b3794 rffM -1.5 -6.7 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b0167 glnD -1.4 -4.4 PII uridylyl-transferase (NCBI) compare
b2297 pta -1.4 -2.3 phosphate acetyltransferase (NCBI) compare
b4481 rffT -1.4 -6.6 4-alpha-L-fucosyltransferase (NCBI) compare
b3619 rfaD -1.4 -4.1 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b1290 sapF -1.4 -2.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4704 arrS -1.4 -1.3 Antisense sRNA ArrS, function unknown (NCBI) compare
b3868 glnG -1.4 -5.4 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b0149 mrcB -1.4 -9.5 penicillin-binding protein 1b (NCBI) compare
b2894 xerD -1.4 -1.8 site-specific tyrosine recombinase XerD (NCBI) compare
b4480 hdfR -1.4 -5.3 transcriptional regulator HdfR (NCBI) compare
b2055 wcaE -1.4 -2.7 predicted glycosyl transferase (NCBI) compare
b2330 yfcB -1.4 -2.7 putative adenine-specific methylase (VIMSS) compare
b1273 yciN -1.4 -1.6 hypothetical protein (NCBI) compare
b3962 udhA -1.4 -7.1 putative oxidoreductase (VIMSS) compare
b1861 ruvA -1.4 -2.8 Holliday junction DNA helicase motor protein (NCBI) compare
b2215 ompC -1.4 -8.3 outer membrane porin protein C (NCBI) compare
b0425 panE -1.3 -6.5 2-dehydropantoate 2-reductase (NCBI) compare
b3839 tatC -1.3 -6.9 TatABCE protein translocation system subunit (NCBI) compare
b4171 miaA -1.3 -5.1 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b3916 pfkA -1.3 -3.4 6-phosphofructokinase (NCBI) compare
b0603 ybdO -1.3 -2.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b2518 ndk -1.3 -2.7 nucleoside diphosphate kinase (NCBI) compare
b4155 yjeA -1.3 -1.4 putative lysyl-tRNA synthetase (VIMSS) compare
b1111 ycfQ -1.3 -3.3 orf, hypothetical protein (VIMSS) compare
b2064 asmA -1.3 -6.1 predicted assembly protein (NCBI) compare
b2574 nadB -1.3 -6.8 L-aspartate oxidase (NCBI) compare
b1192 ldcA -1.3 -4.5 L,D-carboxypeptidase A (NCBI) compare
b3787 rffD -1.3 -7.9 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3204 ptsN -1.3 -4.7 sugar-specific enzyme IIA component of PTS (NCBI) compare
b3790 rffC -1.2 -4.3 TDP-fucosamine acetyltransferase (RefSeq) compare
b0500 ybbD -1.2 -2.3 hypothetical protein (RefSeq) compare
b4178 yjeB -1.2 -4.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2620 smpB -1.2 -1.6 SsrA-binding protein (NCBI) compare
b0778 bioD -1.2 -3.6 dethiobiotin synthetase (NCBI) compare
b0957 ompA -1.2 -4.2 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b1286 rnb -1.2 -3.2 exoribonuclease II (NCBI) compare
b2569 lepA -1.2 -4.0 GTP-binding protein LepA (NCBI) compare
b3791 rffA -1.2 -6.2 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b4025 pgi -1.2 -6.5 glucose-6-phosphate isomerase (NCBI) compare
b3706 trmE -1.2 -4.9 tRNA modification GTPase (NCBI) compare
b1863 ruvC -1.2 -3.3 Holliday junction resolvase (NCBI) compare
b3784 rfe -1.2 -8.5 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b4538 yoeF -1.2 -2.1 hypothetical protein (RefSeq) compare
b3199 yrbK -1.1 -1.5 hypothetical protein (NCBI) compare
b3035 tolC -1.1 -5.7 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b3935 priA -1.1 -1.9 primosome assembly protein PriA (NCBI) compare
b1257 yciE -1.1 -3.0 hypothetical protein (NCBI) compare
b0131 panD -1.1 -3.4 aspartate 1-decarboxylase precursor (NCBI) compare
b0018 mokC -1.1 -1.5 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b3412 bioH -1.1 -5.3 carboxylesterase of pimeloyl-CoA synthesis (NCBI) compare
b0774 bioA -1.1 -3.4 adenosylmethionine--8-amino-7-oxononanoate transaminase (NCBI) compare
b3164 pnp -1.1 -2.9 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4497 yeeL -1.1 -3.6 no description compare
b3786 rffE -1.0 -7.5 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b2822 recC -1.0 -4.0 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b0053 surA -1.0 -4.2 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b1175 minD -1.0 -4.7 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b4146 yjeK -1.0 -2.6 predicted lysine aminomutase (NCBI) compare
b0121 speE -1.0 -4.3 spermidine synthase (NCBI) compare
b0623 cspE -1.0 -2.2 cold shock protein E (NCBI) compare
b4651 yibW -1.0 -1.6 no description compare
b1865 nudB -0.9 -1.6 dATP pyrophosphohydrolase (NCBI) compare
b2904 gcvH -0.9 -2.1 glycine cleavage system protein H (NCBI) compare
b1176 minC -0.9 -3.2 septum formation inhibitor (NCBI) compare
b4375 prfC -0.9 -4.8 peptide chain release factor 3 (NCBI) compare
b0889 lrp -0.9 -2.5 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b1514 lsrC -0.9 -1.8 AI2 transporter (NCBI) compare
b3620 rfaF -0.9 -3.5 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0777 bioC -0.9 -3.8 predicted methltransferase, enzyme of biotin synthesis (NCBI) compare
b4179 vacB -0.9 -5.1 putative enzyme (VIMSS) compare
b2762 cysH -0.9 -4.1 phosphoadenosine phosphosulfate reductase (NCBI) compare
b3021 ygiT -0.9 -2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b0658 ybeX -0.9 -5.1 predicteed ion transport (NCBI) compare
b0866 ybjQ -0.9 -1.2 hypothetical protein (NCBI) compare
b1307 pspD -0.9 -1.9 peripheral inner membrane phage-shock protein (NCBI) compare
b0776 bioF -0.9 -3.8 8-amino-7-oxononanoate synthase (NCBI) compare
b3386 rpe -0.9 -1.5 ribulose-phosphate 3-epimerase (NCBI) compare
b3609 secB -0.9 -3.3 export protein SecB (NCBI) compare
b3404 envZ -0.9 -5.1 osmolarity sensor protein (NCBI) compare
b0088 murD -0.9 -1.5 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b2935 tktA -0.9 -2.3 transketolase 1, thiamin-binding (NCBI) compare
b3026 qseC -0.9 -3.4 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b3792 wzxE -0.9 -4.8 O-antigen translocase (NCBI) compare
b3727 pstC -0.8 -3.6 phosphate transporter subunit (NCBI) compare
b0439 lon -0.8 -3.6 DNA-binding ATP-dependent protease La (NCBI) compare
b4242 mgtA -0.8 -6.6 magnesium transporter (NCBI) compare
b4410 ecnA -0.8 -1.1 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b2017 yefM -0.8 -1.9 orf, hypothetical protein (VIMSS) compare
b1942 fliJ -0.8 -0.9 flagellar biosynthesis chaperone (NCBI) compare
b1704 aroH -0.8 -3.4 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible (NCBI) conserved
b3442 yhhZ -0.8 -2.0 hypothetical protein (NCBI) compare
b1576 ydfD -0.8 -1.2 Qin prophage; predicted protein (NCBI) compare
b4214 cysQ -0.8 -5.8 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b3237 argR -0.8 -2.9 arginine repressor (NCBI) compare
b1426 ydcH -0.8 -1.6 orf, hypothetical protein (VIMSS) compare
b3940 metL -0.8 -5.1 bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI) compare
b2144 sanA -0.8 -6.4 hypothetical protein (NCBI) compare
b3183 obgE -0.8 -1.3 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0627 tatE -0.8 -1.3 twin arginine translocase protein E (NCBI) compare
b0450 glnK -0.8 -1.4 nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB (NCBI) compare
b3290 trkA -0.8 -2.2 potassium transporter peripheral membrane component (NCBI) compare
b3238 yhcN -0.8 -1.7 orf, hypothetical protein (VIMSS) compare
b2424 cysU -0.8 -1.9 sulfate/thiosulfate transporter subunit (NCBI) compare
b0767 ybhE -0.8 -2.6 6-phosphogluconolactonase (NCBI) compare
b3726 pstA -0.8 -3.3 phosphate transporter subunit (NCBI) compare
b0113 pdhR -0.8 -3.1 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b1685 ydiH -0.8 -1.4 predicted protein (RefSeq) compare
b3650 spoT -0.8 -4.1 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b2905 gcvT -0.8 -3.9 glycine cleavage system aminomethyltransferase T (NCBI) compare
b4279 yjhB -0.7 -2.4 putative transport protein (VIMSS) compare
b4436 ryeC -0.7 -1.2 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b3398 yrfF -0.7 -2.2 predicted inner membrane protein (NCBI) compare


Specific Phenotypes

For 5 genes in this experiment

For nitrogen source casamino acids in Escherichia coli BW25113

For nitrogen source casamino acids across organisms