Experiment set1IT093 for Escherichia coli BW25113

casamino acids nitrogen source

Group: nitrogen source
Media: M9 minimal media_noNitrogen + casamino acids (2 mg/ml)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 6.8 generations
By: Kelly on 6/19/2014
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride

Specific Phenotypes

For 5 genes in this experiment

For nitrogen source casamino acids in Escherichia coli BW25113

For nitrogen source casamino acids across organisms

SEED Subsystems

Subsystem	#Specific
Coenzyme A Biosynthesis	3

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pantothenate and CoA biosynthesis
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• C21-Steroid hormone metabolism
• Glycine, serine and threonine metabolism
• Bisphenol A degradation
• beta-Alanine metabolism
• Nucleotide sugars metabolism
• Polyketide sugar unit biosynthesis
• Aminosugars metabolism
• Linoleic acid metabolism
• Tetrachloroethene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Retinol metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide products
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phosphopantothenate biosynthesis I	4	4	3
phosphopantothenate biosynthesis III (archaea)	4	2	2
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	3
superpathway of coenzyme A biosynthesis II (plants)	10	5	3
8-amino-7-oxononanoate biosynthesis I	11	11	1
biotin biosynthesis I	15	15	1