Experiment set1IT092 for Pseudomonas sp. RS175

Guanosine carbon 10 mM

Group: carbon source
Media: MME_noCarbon + Guanosine (10 mM), pH=7
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 1-Jul-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 11 genes in this experiment

For carbon source Guanosine in Pseudomonas sp. RS175

For carbon source Guanosine across organisms

SEED Subsystems

Subsystem	#Specific
Purine Utilization	5
Allantoin Utilization	2
Photorespiration (oxidative C2 cycle)	2
Purine conversions	2
D-galactarate, D-glucarate and D-glycerate catabolism	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Glycine and Serine Utilization	1
Molybdenum cofactor biosynthesis	1
Queuosine-Archaeosine Biosynthesis	1
Serine-glyoxylate cycle	1
Transport of Molybdenum	1
Transport of Zinc	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Glyoxylate and dicarboxylate metabolism
• Glycine, serine and threonine metabolism
• Glycerolipid metabolism
• Nicotinate and nicotinamide metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
guanosine nucleotides degradation II	4	4	3
superpathway of guanosine nucleotides degradation (plants)	6	5	4
guanosine nucleotides degradation III	4	4	2
glycolate and glyoxylate degradation I	4	4	2
adenine and adenosine salvage II	2	2	1
guanine and guanosine salvage II	2	2	1
adenosine nucleotides degradation I	8	7	4
purine nucleotides degradation I (plants)	12	10	6
guanosine nucleotides degradation I	4	3	2
2-O-α-mannosyl-D-glycerate degradation	2	1	1
adenosine nucleotides degradation II	5	5	2
superpathway of purines degradation in plants	18	14	6
superpathway of glycol metabolism and degradation	7	6	2
purine nucleotides degradation II (aerobic)	11	11	3
inosine 5'-phosphate degradation	4	4	1
D-glucarate degradation I	4	3	1
D-galactarate degradation I	4	3	1
L-threonate degradation	4	1	1
D-erythronate degradation II	4	1	1
purine nucleobases degradation II (anaerobic)	24	16	5
superpathway of D-glucarate and D-galactarate degradation	5	4	1
ureide biosynthesis	7	6	1
drosopterin and aurodrosopterin biosynthesis	7	5	1
caffeine degradation III (bacteria, via demethylation)	7	1	1
2-deoxy-D-ribose degradation II	8	4	1
Entner-Doudoroff pathway II (non-phosphorylative)	9	6	1
photorespiration III	9	5	1
photorespiration I	9	5	1
theophylline degradation	9	1	1
photorespiration II	10	6	1
caffeine degradation IV (bacteria, via demethylation and oxidation)	10	1	1
formaldehyde assimilation I (serine pathway)	13	6	1
purine nucleobases degradation I (anaerobic)	15	6	1
superpathway of microbial D-galacturonate and D-glucuronate degradation	31	14	1