Experiment set1IT087 for Escherichia coli BW25113

D-Alanine nitrogen source

Group: nitrogen source
Media: M9 minimal media_noNitrogen + D-Alanine (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 6.0 generations
By: Kelly on 6/19/2014
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride

Specific Phenotypes

For 7 genes in this experiment

For nitrogen source D-Alanine in Escherichia coli BW25113

For nitrogen source D-Alanine across organisms

SEED Subsystems

Subsystem	#Specific
Coenzyme A Biosynthesis	2
Ammonia assimilation	1
Cyanophycin Metabolism	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Pyruvate Alanine Serine Interconversions	1
Respiratory dehydrogenases 1	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pantothenate and CoA biosynthesis
• Nitrogen metabolism
• Alanine and aspartate metabolism
• Phenylalanine metabolism
• beta-Alanine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
superpathway of L-asparagine biosynthesis	2	2	2
L-glutamine degradation I	1	1	1
L-asparagine biosynthesis II	1	1	1
L-asparagine biosynthesis I	1	1	1
phosphopantothenate biosynthesis I	4	4	2
L-glutamate biosynthesis I	2	2	1
L-alanine degradation I	2	2	1
ammonia assimilation cycle III	3	3	1
superpathway of L-aspartate and L-asparagine biosynthesis	4	4	1
phosphopantothenate biosynthesis III (archaea)	4	2	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	1	1
L-asparagine biosynthesis III (tRNA-dependent)	4	1	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	2
superpathway of coenzyme A biosynthesis II (plants)	10	5	2
L-glutamate and L-glutamine biosynthesis	7	5	1
L-citrulline biosynthesis	8	6	1
superpathway of L-citrulline metabolism	12	8	1