Culturing: MS1868_ML3, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
| Pathway | #Steps | #Present | #Specific |
| L-malate degradation II | 1 | 1 | 1 |
| 4-hydroxybenzoate biosynthesis II (bacteria) | 1 | 1 | 1 |
| malate/L-aspartate shuttle pathway | 2 | 2 | 1 |
| 2-deoxy-α-D-ribose 1-phosphate degradation | 3 | 3 | 1 |
| pentose phosphate pathway (oxidative branch) I | 3 | 3 | 1 |
| prenylated FMNH2 biosynthesis | 3 | 3 | 1 |
| L-carnitine degradation II | 3 | 1 | 1 |
| anaerobic energy metabolism (invertebrates, cytosol) | 7 | 5 | 2 |
| chitin deacetylation | 4 | 3 | 1 |
| polybrominated phenols biosynthesis | 4 | 1 | 1 |
| C4 photosynthetic carbon assimilation cycle, NAD-ME type | 11 | 8 | 2 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 10 | 3 |
| purine ribonucleosides degradation | 6 | 6 | 1 |
| glyoxylate cycle | 6 | 6 | 1 |
| superpathway of pyrimidine deoxyribonucleosides degradation | 6 | 6 | 1 |
| TCA cycle VIII (Chlamydia) | 6 | 5 | 1 |
| methylgallate degradation | 6 | 2 | 1 |
| gluconeogenesis I | 13 | 13 | 2 |
| superpathway of purine deoxyribonucleosides degradation | 7 | 7 | 1 |
| incomplete reductive TCA cycle | 7 | 5 | 1 |
| pyruvate fermentation to propanoate I | 7 | 3 | 1 |
| spongiadioxin C biosynthesis | 7 | 1 | 1 |
| mixed acid fermentation | 16 | 16 | 2 |
| pentose phosphate pathway | 8 | 8 | 1 |
| protocatechuate degradation I (meta-cleavage pathway) | 8 | 3 | 1 |
| polybrominated dihydroxylated diphenyl ethers biosynthesis | 8 | 1 | 1 |
| superpathway of glucose and xylose degradation | 17 | 16 | 2 |
| Entner-Doudoroff pathway I | 9 | 9 | 1 |
| Entner-Doudoroff pathway II (non-phosphorylative) | 9 | 7 | 1 |
| TCA cycle II (plants and fungi) | 9 | 7 | 1 |
| TCA cycle IV (2-oxoglutarate decarboxylase) | 9 | 7 | 1 |
| TCA cycle V (2-oxoglutarate synthase) | 9 | 7 | 1 |
| ubiquinol-8 biosynthesis (late decarboxylation) | 9 | 6 | 1 |
| Entner-Doudoroff pathway III (semi-phosphorylative) | 9 | 6 | 1 |
| p-HBAD biosynthesis | 9 | 1 | 1 |
| Rubisco shunt | 10 | 9 | 1 |
| TCA cycle III (animals) | 10 | 8 | 1 |
| glycolysis IV | 10 | 8 | 1 |
| TCA cycle I (prokaryotic) | 10 | 8 | 1 |
| glycolysis V (Pyrococcus) | 10 | 7 | 1 |
| anaerobic energy metabolism (invertebrates, mitochondrial) | 10 | 5 | 1 |
| superpathway of vanillin and vanillate degradation | 10 | 3 | 1 |
| glycolysis III (from glucose) | 11 | 11 | 1 |
| glycolysis II (from fructose 6-phosphate) | 11 | 11 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 18 | 2 |
| glycolysis VI (from fructose) | 11 | 8 | 1 |
| reductive TCA cycle I | 11 | 8 | 1 |
| L-glutamate degradation VIII (to propanoate) | 11 | 3 | 1 |
| superpathway of ubiquinol-8 biosynthesis (early decarboxylation) | 12 | 12 | 1 |
| homolactic fermentation | 12 | 11 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 10 | 1 |
| gluconeogenesis III | 12 | 9 | 1 |
| reductive TCA cycle II | 12 | 8 | 1 |
| syringate degradation | 12 | 3 | 1 |
| glycolysis I (from glucose 6-phosphate) | 13 | 13 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 24 | 2 |
| formaldehyde assimilation I (serine pathway) | 13 | 8 | 1 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 7 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 1 |
| tetrahydromethanopterin biosynthesis | 14 | 3 | 1 |
| Bifidobacterium shunt | 15 | 12 | 1 |
| glycerol degradation to butanol | 16 | 11 | 1 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | 17 | 17 | 1 |
| superpathway of hexitol degradation (bacteria) | 18 | 18 | 1 |
| heterolactic fermentation | 18 | 15 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 18 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 17 | 1 |
| methylaspartate cycle | 19 | 9 | 1 |
| superpathway of polybrominated aromatic compound biosynthesis | 20 | 2 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 22 | 1 |
| phenolphthiocerol biosynthesis | 23 | 1 | 1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 19 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 22 | 1 |
| superpathway of chorismate metabolism | 59 | 54 | 1 |