Experiment set1IT083 for Escherichia coli BW25113
L-Alanine nitrogen source
Group: nitrogen sourceMedia: M9 minimal media_noNitrogen + L-Alanine (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.7 generations
By: Kelly on 6/19/2014
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride
Specific Phenotypes
For 2 genes in this experiment
For nitrogen source L-Alanine in Escherichia coli BW25113
For nitrogen source L-Alanine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Alanine biosynthesis | 1 |
| Pyruvate Alanine Serine Interconversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| alanine racemization | 1 | 1 | 1 |
| L-alanine degradation I | 2 | 2 | 1 |
| anaerobic energy metabolism (invertebrates, cytosol) | 7 | 5 | 1 |
| ansatrienin biosynthesis | 9 | 1 | 1 |
| cyclosporin A biosynthesis | 15 | 2 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 12 | 1 |