Experiment set1IT081 for Escherichia coli BW25113

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Glycine nitrogen source

Group: nitrogen source
Media: M9 minimal media_noNitrogen + Glycine (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.8 generations
By: Kelly on 6/19/2014
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride

Specific Phenotypes

For 5 genes in this experiment

For nitrogen source Glycine in Escherichia coli BW25113

For nitrogen source Glycine across organisms

SEED Subsystems

Subsystem #Specific
Ammonia assimilation 1
Cyanophycin Metabolism 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-serine degradation 3 3 3
superpathway of L-asparagine biosynthesis 2 2 2
cis-cyclopropane fatty acid (CFA) biosynthesis 1 1 1
L-asparagine biosynthesis II 1 1 1
L-asparagine biosynthesis I 1 1 1
L-glutamine degradation I 1 1 1
D-serine degradation 3 3 2
L-tryptophan degradation II (via pyruvate) 3 3 2
L-cysteine degradation II 3 2 2
glutathione degradation (DUG pathway) 2 2 1
L-glutamate biosynthesis I 2 2 1
sterculate biosynthesis 2 1 1
glycine betaine degradation III 7 4 3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
glycine betaine degradation I 8 4 3
glycine degradation 3 3 1
ammonia assimilation cycle III 3 3 1
L-methionine biosynthesis II 6 5 2
superpathway of L-aspartate and L-asparagine biosynthesis 4 4 1
L-mimosine degradation 8 4 2
glutathione-mediated detoxification I 8 3 2
glutaminyl-tRNAgln biosynthesis via transamidation 4 1 1
L-asparagine biosynthesis III (tRNA-dependent) 4 1 1
γ-glutamyl cycle 6 5 1
L-glutamate and L-glutamine biosynthesis 7 5 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 2
L-citrulline biosynthesis 8 6 1
purine nucleobases degradation II (anaerobic) 24 17 3
superpathway of L-citrulline metabolism 12 8 1
mycolate biosynthesis 205 25 3
superpathway of mycolate biosynthesis 239 26 3