Experiment set1IT076 for Pseudomonas sp. RS175

L-Phenylalanine carbon 5 mM

Group: carbon source
Media: MME_noCarbon + L-Phenylalanine (5 mM), pH=7
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 1-Jul-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source L-Phenylalanine in Pseudomonas sp. RS175

For carbon source L-Phenylalanine across organisms

SEED Subsystems

Subsystem	#Specific
Aromatic amino acid degradation	2
Homogentisate pathway of aromatic compound degradation	2
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more.	1
Folate Biosynthesis	1
Plastoquinone Biosynthesis	1
Tocopherol Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis
• Phenylalanine metabolism
• Styrene degradation
• Folate biosynthesis
• Biosynthesis of phenylpropanoids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine degradation I	1	1	1
4-aminobenzoate biosynthesis I	2	2	1
L-glutamate biosynthesis I	2	2	1
L-tyrosine degradation I	5	5	2
ammonia assimilation cycle III	3	3	1
plastoquinol-9 biosynthesis I	3	1	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	4	1
superpathway of plastoquinol biosynthesis	5	2	1
L-glutamate and L-glutamine biosynthesis	7	5	1
vitamin E biosynthesis (tocopherols)	7	1	1
L-citrulline biosynthesis	8	7	1
L-phenylalanine degradation IV (mammalian, via side chain)	9	5	1
chloramphenicol biosynthesis	9	1	1
superpathway of tetrahydrofolate biosynthesis	10	8	1
superpathway of candicidin biosynthesis	11	4	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
superpathway of L-citrulline metabolism	12	9	1
superpathway of chorismate metabolism	59	43	1