Experiment set1IT073 for Escherichia coli BW25113
L-Aspartic Acid nitrogen source
Group: nitrogen sourceMedia: M9 minimal media_noNitrogen + L-Aspartic Acid (6 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.6 generations
By: Kelly on 6/19/2014
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride
Specific Phenotypes
For 2 genes in this experiment
For nitrogen source L-Aspartic Acid in Escherichia coli BW25113
For nitrogen source L-Aspartic Acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cyanophycin Metabolism | 1 |
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| superpathway of L-asparagine biosynthesis | 2 | 2 | 2 |
| L-glutamine degradation I | 1 | 1 | 1 |
| L-asparagine biosynthesis II | 1 | 1 | 1 |
| L-asparagine biosynthesis I | 1 | 1 | 1 |
| L-glutamate biosynthesis I | 2 | 2 | 1 |
| L-aspartate degradation II (aerobic) | 3 | 3 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 1 |
| L-aspartate degradation III (anaerobic) | 3 | 3 | 1 |
| superpathway of L-aspartate and L-asparagine biosynthesis | 4 | 4 | 1 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 1 | 1 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 1 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 5 | 1 |
| L-citrulline biosynthesis | 8 | 6 | 1 |
| superpathway of L-citrulline metabolism | 12 | 8 | 1 |