Experiment set1IT072 for Pontibacter actiniarum KMM 6156, DSM 19842

marine broth with Sodium Chloride 750 mM

Group: stress
Media: marine_broth_2216 + Sodium Chloride (750 mM)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.1 generations
By: Adam on 29-Mar-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1734 D5

Specific Phenotypes

For 18 genes in this experiment

For stress Sodium Chloride in Pontibacter actiniarum KMM 6156, DSM 19842

For stress Sodium Chloride across organisms

SEED Subsystems

Subsystem	#Specific
Proline Synthesis	2
Tryptophan synthesis	2
Auxin biosynthesis	1
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more.	1
De Novo Purine Biosynthesis	1
Fatty Acid Biosynthesis FASII	1
Glycine Biosynthesis	1
Glycine and Serine Utilization	1
Heat shock dnaK gene cluster extended	1
Protein chaperones	1
Pyruvate Alanine Serine Interconversions	1
SigmaB stress responce regulation	1
Threonine degradation	1
Transcription initiation, bacterial sigma factors	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Glycine, serine and threonine metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Propanoate metabolism
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones
• Fatty acid biosynthesis
• Purine metabolism
• Tetracycline biosynthesis
• Valine, leucine and isoleucine degradation
• Benzoate degradation via hydroxylation
• Pyruvate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-serine degradation	3	3	3
L-glutamine degradation I	1	1	1
L-proline biosynthesis I (from L-glutamate)	4	4	3
L-serine degradation	3	3	2
L-ornithine biosynthesis II	3	3	2
L-cysteine degradation II	3	3	2
L-threonine degradation III (to methylglyoxal)	3	2	2
L-tryptophan degradation II (via pyruvate)	3	2	2
L-glutamate biosynthesis I	2	2	1
L-threonine degradation II	2	2	1
aminopropanol phosphate biosynthesis II	4	2	2
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
L-citrulline biosynthesis	8	7	3
L-tryptophan biosynthesis	6	6	2
L-proline biosynthesis III (from L-ornithine)	3	3	1
ammonia assimilation cycle III	3	3	1
propanoyl CoA degradation I	3	3	1
L-methionine biosynthesis II	6	3	2
fatty acid biosynthesis initiation (type I)	3	1	1
L-Nδ-acetylornithine biosynthesis	7	5	2
glycine betaine degradation III	7	4	2
L-arginine degradation VI (arginase 2 pathway)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	4	1
superpathway of L-citrulline metabolism	12	9	3
2-oxobutanoate degradation I	4	3	1
L-asparagine biosynthesis III (tRNA-dependent)	4	3	1
glycine betaine degradation I	8	4	2
L-mimosine degradation	8	4	2
glutathione-mediated detoxification I	8	2	2
acridone alkaloid biosynthesis	4	1	1
protocatechuate degradation II (ortho-cleavage pathway)	4	1	1
5-aminoimidazole ribonucleotide biosynthesis I	5	5	1
5-aminoimidazole ribonucleotide biosynthesis II	5	4	1
L-arginine degradation XIII (reductive Stickland reaction)	5	4	1
4-hydroxy-2(1H)-quinolone biosynthesis	5	2	1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis	6	5	1
superpathway of L-tryptophan biosynthesis	13	13	2
L-glutamate and L-glutamine biosynthesis	7	6	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	11	2
superpathway of aromatic amino acid biosynthesis	18	17	2
superpathway of L-threonine metabolism	18	10	2
jadomycin biosynthesis	9	3	1
aromatic compounds degradation via β-ketoadipate	9	3	1
anaerobic energy metabolism (invertebrates, mitochondrial)	10	6	1
superpathway of quinolone and alkylquinolone biosynthesis	10	2	1
toluene degradation III (aerobic) (via p-cresol)	11	3	1
purine nucleobases degradation II (anaerobic)	24	13	2
3-hydroxypropanoate cycle	13	8	1
superpathway of L-methionine salvage and degradation	16	9	1
superpathway of anaerobic energy metabolism (invertebrates)	17	11	1
3-hydroxypropanoate/4-hydroxybutanate cycle	18	11	1
superpathway of the 3-hydroxypropanoate cycle	18	8	1
methylaspartate cycle	19	11	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	1
superpathway of purine nucleotides de novo biosynthesis II	26	23	1
superpathway of chorismate metabolism	59	39	2
superpathway of aerobic toluene degradation	30	4	1
superpathway of aromatic compound degradation via 3-oxoadipate	35	8	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	42	1
superpathway of mycolate biosynthesis	239	24	2
mycolate biosynthesis	205	23	1