Experiment set1IT072 for Escherichia coli BW25113

L-Arginine nitrogen source

Group: nitrogen source
Media: M9 minimal media_noNitrogen + L-Arginine (10 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.0 generations
By: Kelly on 6/19/2014
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride

Specific Phenotypes

For 13 genes in this experiment

For nitrogen source L-Arginine in Escherichia coli BW25113

For nitrogen source L-Arginine across organisms

SEED Subsystems

Subsystem	#Specific
Arginine and Ornithine Degradation	6
Cyanophycin Metabolism	1
DNA-binding regulatory proteins, strays	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• Fatty acid biosynthesis
• Alanine and aspartate metabolism
• Lysine degradation
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• Ether lipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Butanoate metabolism
• Limonene and pinene degradation
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Nitrogen metabolism
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of terpenoids and steroids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
superpathway of L-asparagine biosynthesis	2	2	2
L-glutamine degradation I	1	1	1
L-asparagine biosynthesis II	1	1	1
L-asparagine biosynthesis I	1	1	1
L-arginine degradation II (AST pathway)	5	5	4
L-glutamate biosynthesis I	2	2	1
ammonia assimilation cycle III	3	3	1
superpathway of L-aspartate and L-asparagine biosynthesis	4	4	1
L-asparagine biosynthesis III (tRNA-dependent)	4	1	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	1	1
L-ornithine biosynthesis I	5	5	1
L-glutamate and L-glutamine biosynthesis	7	5	1
L-citrulline biosynthesis	8	6	1
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
L-arginine biosynthesis II (acetyl cycle)	10	9	1
superpathway of L-citrulline metabolism	12	8	1
superpathway of arginine and polyamine biosynthesis	17	16	1