Experiment set1IT069 for Escherichia coli BW25113
D-Glucose carbon source MOPS
Group: carbon sourceMedia: MOPS Rich Defined media_noCarbon + D-Glucose (22 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.2 generations
By: Kelly on 6/19/2014
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate, 1.32 mM Potassium phosphate dibasic, 1.5 mM Potassium hydroxide, 0.2 mM Adenine hydrochloride hydrate, 0.2 mM Cytosine, 0.2 mM Uracil, 0.2 mM Guanine, 0.8 mM L-Alanine, 5.2 mM L-Arginine, 0.4 mM L-Asparagine, 0.4 mM L-Aspartic acid potassium salt, 0.66 mM L-Glutamic acid monopotassium salt monohydrate, 0.6 mM L-Glutamine, 0.8 mM Glycine, 0.2 mM L-Histidine, 0.4 mM L-Isoleucine, 0.4 mM L-Proline, 10 mM L-Serine, 0.4 mM L-Threonine, 0.1 mM L-Tryptophan, 0.6 mM L-Valine, 0.8 mM L-Leucine, 0.4 mM L-Lysine, 0.2 mM L-Methionine, 0.4 mM L-Phenylalanine, 0.1 mM L-Cysteine hydrochloride monohydrate, 0.2 mM L-tyrosine, 0.01 mM Thiamine HCl, 0.01 mM calcium pantothenate, 0.01 mM 4-Aminobenzoic acid, 0.01 mM 4-Hydroxybenzoic Acid, 0.01 mM 2,3-dihydroxybenzoic acid
Specific Phenotypes
For 6 genes in this experiment
For carbon source D-Glucose in Escherichia coli BW25113
For carbon source D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Biotin biosynthesis | 3 |
| Glycine and Serine Utilization | 1 |
| Pyruvate Alanine Serine Interconversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: