Experiment set1IT067 for Pseudomonas sp. RS175

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N-Acetyl-D-Glucosamine carbon 10 mM

Group: carbon source
Media: MME_noCarbon + N-Acetyl-D-Glucosamine (10 mM), pH=7
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 1-Jul-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source N-Acetyl-D-Glucosamine in Pseudomonas sp. RS175

For carbon source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem #Specific
Chitin and N-acetylglucosamine utilization 2
Sialic Acid Metabolism 2
Fructose and Mannose Inducible PTS 1
Fructose utilization 1
Mannitol Utilization 1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
N-acetylglucosamine degradation I 2 2 1
N-acetylglucosamine degradation II 3 2 1
D-galactosamine and N-acetyl-D-galactosamine degradation 4 2 1
N-acetyl-D-galactosamine degradation 5 2 1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 2 1
chitin derivatives degradation 8 2 1
peptidoglycan recycling I 14 11 1
superpathway of N-acetylneuraminate degradation 22 15 1