Experiment set1IT061 for Escherichia coli BW25113

N-Acetyl-D-Glucosamine carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + N-Acetyl-D-Glucosamine (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.9 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 6 genes in this experiment

For carbon source N-Acetyl-D-Glucosamine in Escherichia coli BW25113

For carbon source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem	#Specific
Chitin and N-acetylglucosamine utilization	2
Coenzyme A Biosynthesis	2
Sialic Acid Metabolism	2
Acetoin, butanediol metabolism	1
Branched-Chain Amino Acid Biosynthesis	1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pantothenate and CoA biosynthesis
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycolysis / Gluconeogenesis
• Fructose and mannose metabolism
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Valine, leucine and isoleucine biosynthesis
• beta-Alanine metabolism
• Starch and sucrose metabolism
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phosphopantothenate biosynthesis I	4	4	2
N-acetylglucosamine degradation I	2	2	1
pyruvate fermentation to (R)-acetoin II	2	1	1
N-acetylglucosamine degradation II	3	3	1
pyruvate fermentation to (S)-acetoin	3	3	1
pyruvate fermentation to (R)-acetoin I	3	2	1
L-valine biosynthesis	4	4	1
phosphopantothenate biosynthesis III (archaea)	4	2	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	2
pyruvate fermentation to isobutanol (engineered)	5	4	1
superpathway of coenzyme A biosynthesis II (plants)	10	5	2
superpathway of (R,R)-butanediol biosynthesis	5	2	1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation	6	6	1
L-isoleucine biosynthesis IV	6	4	1
superpathway of 2,3-butanediol biosynthesis	6	3	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
UDP-N-acetyl-D-galactosamine biosynthesis II	7	5	1
L-isoleucine biosynthesis III	7	4	1
chitin degradation I (archaea)	7	2	1
chitin derivatives degradation	8	4	1
L-isoleucine biosynthesis II	8	4	1
superpathway of branched chain amino acid biosynthesis	17	17	2
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of L-threonine metabolism	18	16	1
superpathway of N-acetylneuraminate degradation	22	22	1