Experiment set1IT060 for Pseudomonas sp. RS175

Inosine carbon 10 mM

Group: carbon source
Media: MME_noCarbon + Inosine (10 mM), pH=7
Culturing: Pseudomonas_RS175_ML2, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 1-Jul-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 24 genes in this experiment

For carbon source Inosine in Pseudomonas sp. RS175

For carbon source Inosine across organisms

SEED Subsystems

Subsystem	#Specific
Purine Utilization	3
Terminal cytochrome C oxidases	3
DNA repair, bacterial MutL-MutS system	2
Purine conversions	2
Allantoin Utilization	1
Coenzyme A Biosynthesis	1
DNA-replication	1
Fructose utilization	1
Mannitol Utilization	1
Photorespiration (oxidative C2 cycle)	1
Polyamine Metabolism	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Tyrosine metabolism
• Tryptophan metabolism
• Phenylpropanoid biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• C21-Steroid hormone metabolism
• Oxidative phosphorylation
• Alanine and aspartate metabolism
• Glycine, serine and threonine metabolism
• Histidine metabolism
• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• Phenylalanine metabolism
• Benzoate degradation via hydroxylation
• Bisphenol A degradation
• Benzoxazinone biosynthesis
• beta-Alanine metabolism
• Nucleotide sugars metabolism
• Polyketide sugar unit biosynthesis
• Aminosugars metabolism
• Lipopolysaccharide biosynthesis
• Linoleic acid metabolism
• Pyruvate metabolism
• Biphenyl degradation
• 1- and 2-Methylnaphthalene degradation
• Tetrachloroethene degradation
• Naphthalene and anthracene degradation
• Fluorene degradation
• Glyoxylate and dicarboxylate metabolism
• Benzoate degradation via CoA ligation
• 3-Chloroacrylic acid degradation
• Butanoate metabolism
• Pantothenate and CoA biosynthesis
• Folate biosynthesis
• Retinol metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide products

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
β-alanine biosynthesis III	1	1	1
adenosine nucleotides degradation III	1	1	1
xanthine and xanthosine salvage	2	2	1
guanine and guanosine salvage I	2	2	1
guanine and guanosine salvage II	2	2	1
arsenite to oxygen electron transfer	2	1	1
adenosine nucleotides degradation II	5	5	2
superpathway of guanine and guanosine salvage	3	2	1
adenine salvage	3	2	1
arsenite to oxygen electron transfer (via azurin)	3	1	1
adenine and adenosine salvage III	4	4	1
glycolate and glyoxylate degradation I	4	4	1
guanosine nucleotides degradation III	4	4	1
inosine 5'-phosphate degradation	4	4	1
guanosine nucleotides degradation II	4	4	1
adenosine nucleotides degradation I	8	7	2
aerobic respiration II (cytochrome c) (yeast)	4	3	1
spermidine biosynthesis II	4	3	1
guanosine nucleotides degradation I	4	3	1
D-fructuronate degradation	4	3	1
aerobic respiration I (cytochrome c)	4	3	1
superpathway of polyamine biosynthesis III	8	5	2
mannitol cycle	5	4	1
purine nucleotides degradation II (aerobic)	11	11	2
purine nucleotides degradation I (plants)	12	10	2
superpathway of guanosine nucleotides degradation (plants)	6	5	1
purine nucleobases degradation II (anaerobic)	24	16	4
norspermidine biosynthesis	6	4	1
Fe(II) oxidation	6	3	1
superpathway of glycol metabolism and degradation	7	6	1
ureide biosynthesis	7	6	1
superpathway of β-D-glucuronosides degradation	7	4	1
caffeine degradation III (bacteria, via demethylation)	7	1	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	1
superpathway of purines degradation in plants	18	14	2
theophylline degradation	9	1	1
superpathway of hexuronide and hexuronate degradation	10	4	1
caffeine degradation IV (bacteria, via demethylation and oxidation)	10	1	1
superpathway of purine nucleotide salvage	14	13	1
superpathway of microbial D-galacturonate and D-glucuronate degradation	31	14	1