Experiment set1IT055 for Magnetospirillum magneticum AMB-1
carbonsource_succinate
Group: carbon sourceMedia: MG minimal media_noCarbon + Succinic Acid (3 mM)
Culturing: Magneto_ML2, tube, Microaerobic, at 30 (C)
By: Hayley on 22-Oct-2018
Media components: 0.035 g/L L-ascorbic acid, 0.1 g/L Sodium thiosulfate, 0.12 g/L Sodium nitrate, 0.68 g/L Potassium phosphate monobasic, Modified Wolfe's Minerals (0.0075 g/L Nitrilotriacetic acid, 0.007325 g/L Magnesium sulfate, 0.0025 g/L Manganese (II) sulfate monohydrate, 0.005 g/L Sodium Chloride, 0.0005 g/L Cobalt chloride hexahydrate, 0.0005 g/L Calcium chloride dihydrate, 0.0005 g/L Zinc sulfate heptahydrate, 0.0001 g/L Aluminum potassium sulfate dodecahydrate, 5e-05 g/L Boric Acid, 5e-05 g/L Sodium Molybdate Dihydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin), ferric malate solution (0.00486 mg/L Ferric chloride, 0.01207 mg/L D,L-Malic Acid)
Specific Phenotypes
For 3 genes in this experiment
For carbon source Succinic Acid in Magnetospirillum magneticum AMB-1
For carbon source Succinic Acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Calvin-Benson cycle | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| Rubisco shunt | 10 | 10 | 1 |
| Calvin-Benson-Bassham cycle | 13 | 12 | 1 |
| oxygenic photosynthesis | 17 | 12 | 1 |
| ethene biosynthesis V (engineered) | 25 | 18 | 1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 19 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 20 | 1 |