Experiment set1IT055 for Escherichia coli BW25113

D-Trehalose dihydrate carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + D-Trehalose dihydrate (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.7 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 3 genes in this experiment

For carbon source D-Trehalose dihydrate in Escherichia coli BW25113

For carbon source D-Trehalose dihydrate across organisms

SEED Subsystems

Subsystem	#Specific
Trehalose Uptake and Utilization	2

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Fructose and mannose metabolism
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-mannose degradation I	2	2	1
glycolysis I (from glucose 6-phosphate)	13	13	1
superpathway of glycolysis and the Entner-Doudoroff pathway	17	17	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	25	1