Experiment set1IT054 for Agrobacterium fabrum C58

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L-Arabinose carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + L-Arabinose (10 mM)
Culturing: Agro_ML11, 24-well transparent microplate; Multitron, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 8/25/20
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 10 genes in this experiment

For carbon source L-Arabinose in Agrobacterium fabrum C58

For carbon source L-Arabinose across organisms

SEED Subsystems

Subsystem #Specific
L-Arabinose utilization 2
D-Galacturonate and D-Glucuronate Utilization 1
Hfl operon 1
Proline, 4-hydroxyproline uptake and utilization 1
RNA processing and degradation, bacterial 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-galactose degradation II 2 2 1
pyruvate fermentation to acetate VIII 2 1 1
D-glucarate degradation II 3 3 1
D-galactarate degradation II 3 3 1
L-carnitine degradation II 3 2 1
L-lyxonate degradation 3 1 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 6 2
1,2-dichloroethane degradation 4 3 1
trans-4-hydroxy-L-proline degradation II 4 2 1
tRNA processing 10 10 2
D-galacturonate degradation II 5 5 1
D-glucuronate degradation II 5 5 1
cytosolic NADPH production (yeast) 5 4 1
mitochondrial NADPH production (yeast) 5 3 1
D-xylose degradation III 5 2 1
D-xylose degradation V 5 2 1
D-arabinose degradation III 6 3 1
L-arabinose degradation III 6 2 1
superpathway of pentose and pentitol degradation 42 16 2
superpathway of microbial D-galacturonate and D-glucuronate degradation 31 22 1