Experiment set1IT051 for Escherichia coli BW25113

D-Sorbitol carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + D-Sorbitol (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.9 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 2 genes in this experiment

For carbon source D-Sorbitol in Escherichia coli BW25113

For carbon source D-Sorbitol across organisms

SEED Subsystems

Subsystem	#Specific
D-Sorbitol(D-Glucitol) and L-Sorbose Utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-sorbitol degradation II	1	1	1
L-sorbose degradation	2	1	1
superpathway of hexitol degradation (bacteria)	18	18	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	19	1