Experiment set1IT047 for Serratia liquefaciens MT49
Cadmium acetate dihydrate 40 uM, aerobic
Group: stressMedia: DefinedMedia_AdamsLab_Glucose_YeastExtract + Cadmium acetate dihydrate (40 uM), pH=7
Culturing: MT049_ML3, hungate, Aerobic, at 24 (C), shaken=150 rpm
Growth: about 6.2 generations
By: Mike Thorgersen on 6/4/19
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 5 mM Sodium phosphate monobasic monohydrate, 2.5 g/L Sodium bicarbonate, 20 mM D-Glucose, 1 g/L Yeast Extract, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 7 genes in this experiment
For stress Cadmium acetate dihydrate in Serratia liquefaciens MT49
For stress Cadmium acetate dihydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Ton and Tol transport systems | 1 |
| Transcription factors bacterial | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid biosynthesis
- Ubiquinone and menaquinone biosynthesis
- alpha-Linolenic acid metabolism
- Benzoate degradation via CoA ligation
- Limonene and pinene degradation
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: