Experiment set1IT046 for Escherichia coli BW25113

D-Galacturonic Acid monohydrate carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + D-Galacturonic Acid monohydrate (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 6.4 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 9 genes in this experiment

For carbon source D-Galacturonic Acid monohydrate in Escherichia coli BW25113

For carbon source D-Galacturonic Acid monohydrate across organisms

SEED Subsystems

Subsystem	#Specific
D-Galacturonate and D-Glucuronate Utilization	3
Alginate metabolism	1
D-gluconate and ketogluconates metabolism	1
Entner-Doudoroff Pathway	1
Lactose and Galactose Uptake and Utilization	1
Leucine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions
• Galactose metabolism
• Nucleotide sugars metabolism
• Pentose phosphate pathway
• Glycine, serine and threonine metabolism
• Glycerolipid metabolism
• Glyoxylate and dicarboxylate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-galacturonate degradation I	5	5	3
2-O-α-mannosyl-D-glycerate degradation	2	2	1
superpathway of hexuronide and hexuronate degradation	10	10	4
β-D-glucuronide and D-glucuronate degradation	3	3	1
D-galactose detoxification	3	3	1
stachyose degradation	7	7	2
superpathway of β-D-glucuronosides degradation	7	7	2
D-fructuronate degradation	4	4	1
D-galactarate degradation I	4	4	1
glycolate and glyoxylate degradation I	4	4	1
D-glucarate degradation I	4	4	1
D-galactose degradation I (Leloir pathway)	5	5	1
superpathway of D-glucarate and D-galactarate degradation	5	5	1
4-deoxy-L-threo-hex-4-enopyranuronate degradation	5	4	1
pectin degradation II	5	3	1
superpathway of microbial D-galacturonate and D-glucuronate degradation	31	19	5
superpathway of glycol metabolism and degradation	7	7	1
3,6-anhydro-α-L-galactopyranose degradation	7	4	1
alginate degradation	7	1	1
2-deoxy-D-ribose degradation II	8	3	1
Entner-Doudoroff pathway III (semi-phosphorylative)	9	6	1
Entner-Doudoroff pathway II (non-phosphorylative)	9	6	1
photorespiration III	9	5	1
photorespiration I	9	5	1
photorespiration II	10	6	1
formaldehyde assimilation I (serine pathway)	13	8	1