Experiment set1IT036 for Escherichia coli BW25113

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Sodium D,L-Lactate carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + Sodium D,L-Lactate (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.1 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 4 genes in this experiment

For carbon source Sodium D,L-Lactate in Escherichia coli BW25113

For carbon source Sodium D,L-Lactate across organisms

SEED Subsystems

Subsystem #Specific
Lactate utilization 2
Alanine biosynthesis 1
Branched-Chain Amino Acid Biosynthesis 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Pyruvate Alanine Serine Interconversions 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine biosynthesis I 2 2 1
superpathway of L-alanine biosynthesis 4 4 1
glycolysis V (Pyrococcus) 10 7 1
glycolysis II (from fructose 6-phosphate) 11 11 1
glycolysis I (from glucose 6-phosphate) 13 13 1
gluconeogenesis I 13 13 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 17 1
superpathway of hexitol degradation (bacteria) 18 18 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 19 1
superpathway of anaerobic sucrose degradation 19 17 1
superpathway of N-acetylneuraminate degradation 22 22 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 25 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 22 1