Experiment set1IT027 for Escherichia coli BW25113

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Potassium acetate carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + Potassium acetate (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 4.8 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 26 genes in this experiment

For carbon source Potassium acetate in Escherichia coli BW25113

For carbon source Potassium acetate across organisms

SEED Subsystems

Subsystem #Specific
Pyruvate metabolism I: anaplerotic reactions, PEP 2
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 1
Autoinducer 2 (AI-2) transport and processing (lsrACDBFGE operon) 1
CBSS-562.2.peg.5158 SK3 including 1
D-ribose utilization 1
Deoxyribose and Deoxynucleoside Catabolism 1
Flavodoxin 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Ketoisovalerate oxidoreductase 1
Methionine Biosynthesis 1
Methionine Degradation 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
Serine-glyoxylate cycle 1
Staphylococcal pathogenicity islands SaPI 1
Trehalose Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-malate degradation I 1 1 1
thiosulfate disproportionation IV (rhodanese) 1 1 1
acetate conversion to acetyl-CoA 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
trehalose biosynthesis I 2 2 1
polyphosphate metabolism 2 2 1
ribose phosphorylation 2 2 1
superpathway of acetate utilization and formation 3 3 1
autoinducer AI-2 degradation 3 3 1
ethanol degradation II 3 3 1
2-deoxy-D-ribose degradation I 3 3 1
ethanol degradation IV 3 3 1
L-isoleucine biosynthesis V 3 2 1
ethanol degradation III 3 2 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 2
chitin deacetylation 4 3 1
2-methylcitrate cycle I 5 5 1
sulfide oxidation IV (mitochondria) 5 2 1
thiazole component of thiamine diphosphate biosynthesis I 6 6 1
glyoxylate cycle 6 6 1
ppGpp metabolism 6 6 1
L-isoleucine biosynthesis IV 6 4 1
2-methylcitrate cycle II 6 4 1
methylgallate degradation 6 2 1
superpathway of bitter acids biosynthesis 18 3 3
lupulone and humulone biosynthesis 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
β-alanine biosynthesis II 6 1 1
gluconeogenesis I 13 13 2
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 2
protocatechuate degradation I (meta-cleavage pathway) 8 3 1
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
cis-geranyl-CoA degradation 9 1 1
superpathway of thiamine diphosphate biosynthesis I 10 10 1
glycolysis V (Pyrococcus) 10 7 1
superpathway of coenzyme A biosynthesis II (plants) 10 5 1
superpathway of vanillin and vanillate degradation 10 3 1
glycolysis II (from fructose 6-phosphate) 11 11 1
superpathway of glyoxylate bypass and TCA 12 11 1
syringate degradation 12 3 1
glycolysis I (from glucose 6-phosphate) 13 13 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 25 2
superpathway of glyoxylate cycle and fatty acid degradation 14 11 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 17 1
superpathway of hexitol degradation (bacteria) 18 18 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 19 1
superpathway of anaerobic sucrose degradation 19 17 1
superpathway of N-acetylneuraminate degradation 22 22 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 22 1