Experiment set1IT025 for Escherichia coli BW25113

D-Glucose-6-Phosphate sodium salt carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + D-Glucose-6-Phosphate sodium salt (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 7.2 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 5 genes in this experiment

For carbon source D-Glucose-6-Phosphate sodium salt in Escherichia coli BW25113

For carbon source D-Glucose-6-Phosphate sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Hexose Phosphate Uptake System	2
Acetoin, butanediol metabolism	1
Arginine Biosynthesis extended	1
Branched-Chain Amino Acid Biosynthesis	1
Lysine Biosynthesis DAP Pathway	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lysine biosynthesis
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine biosynthesis
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyruvate fermentation to (R)-acetoin II	2	1	1
pyruvate fermentation to (S)-acetoin	3	3	1
pyruvate fermentation to (R)-acetoin I	3	2	1
L-valine biosynthesis	4	4	1
L-arginine degradation II (AST pathway)	5	5	1
L-ornithine biosynthesis I	5	5	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
superpathway of (R,R)-butanediol biosynthesis	5	2	1
thiazole component of thiamine diphosphate biosynthesis I	6	6	1
L-isoleucine biosynthesis IV	6	4	1
superpathway of 2,3-butanediol biosynthesis	6	3	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
L-isoleucine biosynthesis III	7	4	1
L-isoleucine biosynthesis II	8	4	1
superpathway of branched chain amino acid biosynthesis	17	17	2
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-lysine biosynthesis I	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
superpathway of thiamine diphosphate biosynthesis I	10	10	1
L-arginine biosynthesis II (acetyl cycle)	10	9	1
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of arginine and polyamine biosynthesis	17	16	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	1
superpathway of L-threonine metabolism	18	16	1
aspartate superpathway	25	24	1