Culturing: Variovorax_OAS795_ML2, 96 deep-well microplate; 0.8 mL volume, Aerobic, at 30 (C), shaken=700 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| fatty acid β-oxidation III (unsaturated, odd number) | 1 | 1 | 1 |
| cis-cyclopropane fatty acid (CFA) biosynthesis | 1 | 1 | 1 |
| adenosine nucleotides degradation III | 1 | 1 | 1 |
| 3-(4-hydroxyphenyl)pyruvate biosynthesis | 1 | 1 | 1 |
| benzoyl-CoA biosynthesis | 3 | 3 | 2 |
| adipate degradation | 5 | 5 | 3 |
| adipate biosynthesis | 5 | 5 | 3 |
| oleate β-oxidation (thioesterase-dependent, yeast) | 2 | 2 | 1 |
| 3-oxoadipate degradation | 2 | 2 | 1 |
| L-alanine biosynthesis I | 2 | 2 | 1 |
| L-isoleucine degradation I | 6 | 4 | 3 |
| L-tyrosine degradation II | 2 | 1 | 1 |
| sterculate biosynthesis | 2 | 1 | 1 |
| atromentin biosynthesis | 2 | 1 | 1 |
| oleate β-oxidation | 35 | 29 | 16 |
| fatty acid β-oxidation I (generic) | 7 | 5 | 3 |
| glutaryl-CoA degradation | 5 | 3 | 2 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 2 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 3 | 2 |
| fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) | 5 | 2 | 2 |
| L-valine degradation I | 8 | 6 | 3 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 4 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 4 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 10 | 5 |
| glyoxylate cycle | 6 | 6 | 2 |
| L-phenylalanine biosynthesis I | 3 | 3 | 1 |
| L-tyrosine biosynthesis I | 3 | 3 | 1 |
| fatty acid salvage | 6 | 5 | 2 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 2 |
| L-phenylalanine degradation II (anaerobic) | 3 | 2 | 1 |
| L-valine degradation II | 3 | 2 | 1 |
| L-leucine degradation III | 3 | 2 | 1 |
| L-isoleucine degradation II | 3 | 2 | 1 |
| L-isoleucine biosynthesis V | 3 | 2 | 1 |
| valproate β-oxidation | 9 | 5 | 3 |
| propanoate fermentation to 2-methylbutanoate | 6 | 3 | 2 |
| methyl ketone biosynthesis (engineered) | 6 | 3 | 2 |
| L-leucine degradation V (oxidative Stickland reaction) | 3 | 1 | 1 |
| oleate β-oxidation (reductase-dependent, yeast) | 3 | 1 | 1 |
| L-tyrosine degradation IV (to 4-methylphenol) | 3 | 1 | 1 |
| L-valine degradation III (oxidative Stickland reaction) | 3 | 1 | 1 |
| L-isoleucine degradation III (oxidative Stickland reaction) | 3 | 1 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 6 | 2 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 4 |
| pyruvate fermentation to butanoate | 7 | 4 | 2 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 3 | 2 |
| toluene degradation III (aerobic) (via p-cresol) | 11 | 7 | 3 |
| superpathway of L-alanine biosynthesis | 4 | 4 | 1 |
| L-valine biosynthesis | 4 | 4 | 1 |
| protocatechuate degradation II (ortho-cleavage pathway) | 4 | 4 | 1 |
| nitrogen remobilization from senescing leaves | 8 | 7 | 2 |
| partial TCA cycle (obligate autotrophs) | 8 | 7 | 2 |
| L-phenylalanine degradation III | 4 | 2 | 1 |
| 4-chlorobenzoate degradation | 4 | 2 | 1 |
| 4-sulfocatechol degradation | 4 | 2 | 1 |
| L-tyrosine degradation III | 4 | 2 | 1 |
| pyruvate fermentation to butanol I | 8 | 3 | 2 |
| 4-methylphenol degradation to protocatechuate | 4 | 1 | 1 |
| oleate β-oxidation (isomerase-dependent, yeast) | 4 | 1 | 1 |
| TCA cycle II (plants and fungi) | 9 | 9 | 2 |
| TCA cycle V (2-oxoglutarate synthase) | 9 | 9 | 2 |
| aromatic compounds degradation via β-ketoadipate | 9 | 8 | 2 |
| TCA cycle IV (2-oxoglutarate decarboxylase) | 9 | 8 | 2 |
| phenylacetate degradation I (aerobic) | 9 | 8 | 2 |
| TCA cycle VI (Helicobacter) | 9 | 7 | 2 |
| TCA cycle VII (acetate-producers) | 9 | 7 | 2 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 6 | 2 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 5 | 2 |
| 4-hydroxybenzoate biosynthesis III (plants) | 5 | 5 | 1 |
| (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) | 5 | 5 | 1 |
| L-tyrosine degradation I | 5 | 5 | 1 |
| 4-coumarate degradation (aerobic) | 5 | 5 | 1 |
| TCA cycle III (animals) | 10 | 9 | 2 |
| TCA cycle I (prokaryotic) | 10 | 9 | 2 |
| glycolysis V (Pyrococcus) | 10 | 7 | 2 |
| 3-phenylpropanoate degradation | 10 | 5 | 2 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 5 | 2 |
| 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) | 10 | 4 | 2 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 2 | 1 |
| superpathway of plastoquinol biosynthesis | 5 | 2 | 1 |
| bisphenol A degradation | 5 | 1 | 1 |
| L-tyrosine degradation V (reductive Stickland reaction) | 5 | 1 | 1 |
| 4-hydroxybenzoate biosynthesis I (eukaryotes) | 5 | 1 | 1 |
| L-phenylalanine degradation VI (reductive Stickland reaction) | 5 | 1 | 1 |
| L-leucine degradation IV (reductive Stickland reaction) | 5 | 1 | 1 |
| glycerol degradation to butanol | 16 | 10 | 3 |
| glycolysis II (from fructose 6-phosphate) | 11 | 11 | 2 |
| superpathway of phenylethylamine degradation | 11 | 9 | 2 |
| reductive TCA cycle I | 11 | 8 | 2 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 3 |
| L-leucine biosynthesis | 6 | 6 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 11 | 2 |
| L-leucine degradation I | 6 | 5 | 1 |
| catechol degradation III (ortho-cleavage pathway) | 6 | 5 | 1 |
| 4-hydroxymandelate degradation | 6 | 4 | 1 |
| L-isoleucine biosynthesis IV | 6 | 4 | 1 |
| reductive TCA cycle II | 12 | 7 | 2 |
| 6-gingerol analog biosynthesis (engineered) | 6 | 3 | 1 |
| L-glutamate degradation VII (to butanoate) | 12 | 5 | 2 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 24 | 4 |
| glycolysis I (from glucose 6-phosphate) | 13 | 12 | 2 |
| gluconeogenesis I | 13 | 11 | 2 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 5 | 2 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 1 |
| superpathway of salicylate degradation | 7 | 6 | 1 |
| 4-methylcatechol degradation (ortho cleavage) | 7 | 4 | 1 |
| L-isoleucine biosynthesis III | 7 | 4 | 1 |
| spongiadioxin C biosynthesis | 7 | 1 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 20 | 3 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 3 |
| L-tryptophan degradation III (eukaryotic) | 15 | 7 | 2 |
| mixed acid fermentation | 16 | 12 | 2 |
| L-isoleucine biosynthesis II | 8 | 6 | 1 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 4 | 2 |
| 2-methylpropene degradation | 8 | 2 | 1 |
| polybrominated dihydroxylated diphenyl ethers biosynthesis | 8 | 1 | 1 |
| ethene biosynthesis V (engineered) | 25 | 19 | 3 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | 17 | 16 | 2 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 8 | 2 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 5 | 2 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 2 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 20 | 3 |
| superpathway of hexitol degradation (bacteria) | 18 | 13 | 2 |
| gluconeogenesis II (Methanobacterium thermoautotrophicum) | 18 | 12 | 2 |
| Entner-Doudoroff pathway III (semi-phosphorylative) | 9 | 6 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 11 | 2 |
| L-phenylalanine degradation IV (mammalian, via side chain) | 9 | 4 | 1 |
| toluene degradation VI (anaerobic) | 18 | 4 | 2 |
| superpathway of anaerobic sucrose degradation | 19 | 16 | 2 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 15 | 2 |
| methylaspartate cycle | 19 | 12 | 2 |
| L-histidine biosynthesis | 10 | 10 | 1 |
| Rubisco shunt | 10 | 10 | 1 |
| superpathway of L-phenylalanine biosynthesis | 10 | 10 | 1 |
| superpathway of L-tyrosine biosynthesis | 10 | 10 | 1 |
| glycolysis IV | 10 | 8 | 1 |
| photorespiration II | 10 | 6 | 1 |
| superpathway of aerobic toluene degradation | 30 | 16 | 3 |
| methyl tert-butyl ether degradation | 10 | 3 | 1 |
| rosmarinic acid biosynthesis I | 10 | 3 | 1 |
| glycolysis III (from glucose) | 11 | 11 | 1 |
| glycolysis VI (from fructose) | 11 | 8 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 15 | 2 |
| gallate degradation III (anaerobic) | 11 | 3 | 1 |
| (S)-reticuline biosynthesis I | 11 | 1 | 1 |
| superpathway of aromatic compound degradation via 3-oxoadipate | 35 | 25 | 3 |
| homolactic fermentation | 12 | 11 | 1 |
| 10-cis-heptadecenoyl-CoA degradation (yeast) | 12 | 2 | 1 |
| 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) | 12 | 2 | 1 |
| androstenedione degradation I (aerobic) | 25 | 6 | 2 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 21 | 2 |
| platensimycin biosynthesis | 26 | 6 | 2 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 2 | 1 |
| androstenedione degradation II (anaerobic) | 27 | 4 | 2 |
| superpathway of rosmarinic acid biosynthesis | 14 | 4 | 1 |
| superpathway of testosterone and androsterone degradation | 28 | 7 | 2 |
| superpathway of cholesterol degradation I (cholesterol oxidase) | 42 | 8 | 3 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 2 | 1 |
| Bifidobacterium shunt | 15 | 14 | 1 |
| superpathway of cholesterol degradation II (cholesterol dehydrogenase) | 47 | 9 | 3 |
| superpathway of glucose and xylose degradation | 17 | 14 | 1 |
| cholesterol degradation to androstenedione I (cholesterol oxidase) | 17 | 2 | 1 |
| superpathway of L-threonine metabolism | 18 | 15 | 1 |
| mandelate degradation to acetyl-CoA | 18 | 14 | 1 |
| cholesterol degradation to androstenedione II (cholesterol dehydrogenase) | 22 | 3 | 1 |
| superpathway of cholesterol degradation III (oxidase) | 49 | 5 | 2 |
| anaerobic aromatic compound degradation (Thauera aromatica) | 27 | 7 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 24 | 2 |
| superpathway of chorismate metabolism | 59 | 40 | 2 |
| anteiso-branched-chain fatty acid biosynthesis | 34 | 31 | 1 |
| odd iso-branched-chain fatty acid biosynthesis | 34 | 31 | 1 |
| even iso-branched-chain fatty acid biosynthesis | 34 | 31 | 1 |
| superpathway of aromatic compound degradation via 2-hydroxypentadienoate | 42 | 22 | 1 |
| superpathway of histidine, purine, and pyrimidine biosynthesis | 46 | 43 | 1 |
| mycolate biosynthesis | 205 | 26 | 3 |
| superpathway of mycolate biosynthesis | 239 | 27 | 3 |