Experiment set1IT024 for Escherichia coli BW25113

D-Gluconic Acid sodium salt carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + D-Gluconic Acid sodium salt (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 6.5 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 3 genes in this experiment

For carbon source D-Gluconic Acid sodium salt in Escherichia coli BW25113

For carbon source D-Gluconic Acid sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
D-gluconate and ketogluconates metabolism	1
Entner-Doudoroff Pathway	1
Methylglyoxal Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Pyruvate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
Entner-Doudoroff shunt	2	2	1
Entner-Doudoroff pathway I	9	9	1
superpathway of glycolysis and the Entner-Doudoroff pathway	17	17	1