Experiment set1IT023 for Escherichia coli BW25113
D-Gluconic Acid sodium salt carbon source
Group: carbon sourceMedia: M9 minimal media_noCarbon + D-Gluconic Acid sodium salt (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 6.5 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride
Specific Phenotypes
For 6 genes in this experiment
For carbon source D-Gluconic Acid sodium salt in Escherichia coli BW25113
For carbon source D-Gluconic Acid sodium salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| D-gluconate and ketogluconates metabolism | 2 |
| Entner-Doudoroff Pathway | 2 |
| Methylglyoxal Metabolism | 1 |
| Pentose phosphate pathway | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Glutathione metabolism
- Lipopolysaccharide biosynthesis
- Pyruvate metabolism
- Nicotinate and nicotinamide metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-gluconate degradation | 1 | 1 | 1 |
| Entner-Doudoroff shunt | 2 | 2 | 1 |
| pentose phosphate pathway (oxidative branch) I | 3 | 3 | 1 |
| L-idonate degradation | 3 | 3 | 1 |
| sorbitol biosynthesis II | 3 | 2 | 1 |
| thiazole component of thiamine diphosphate biosynthesis I | 6 | 6 | 1 |
| ketogluconate metabolism | 8 | 8 | 1 |
| pentose phosphate pathway | 8 | 8 | 1 |
| Entner-Doudoroff pathway I | 9 | 9 | 1 |
| superpathway of thiamine diphosphate biosynthesis I | 10 | 10 | 1 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | 17 | 17 | 1 |
| superpathway of glucose and xylose degradation | 17 | 17 | 1 |