Experiment set1IT023 for Echinicola vietnamensis KMM 6221, DSM 17526
marine broth with copper (II) chloride dihydrate 0.5 mM
Group: stressMedia: marine_broth_2216 + copper (II) chloride dihydrate (0.5 mM)
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.0 generations
By: Adam on 29-Mar-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1727 B1
Specific Phenotypes
For 20 genes in this experiment
For stress copper (II) chloride dihydrate in Echinicola vietnamensis KMM 6221, DSM 17526
For stress copper (II) chloride dihydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cobalt-zinc-cadmium resistance | 2 |
| Terminal cytochrome C oxidases | 2 |
| Oxidative stress | 1 |
| Sialic Acid Metabolism | 1 |
| Ton and Tol transport systems | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| arsenite to oxygen electron transfer | 2 | 1 | 1 |
| arsenite to oxygen electron transfer (via azurin) | 3 | 1 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 3 | 1 |
| aerobic respiration II (cytochrome c) (yeast) | 4 | 2 | 1 |
| Fe(II) oxidation | 6 | 2 | 1 |