Experiment set1IT022 for Pseudomonas syringae pv. syringae B728a ΔmexB

KB with Erythromycin 6.25 ug/ml

Group: stress
Media: KB + Erythromycin (6.25 ug/ml)
Culturing: SyringaeB728a_mexBdelta_ML3, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler on 12/4/17
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 24 genes in this experiment

For stress Erythromycin in Pseudomonas syringae pv. syringae B728a ΔmexB

For stress Erythromycin across organisms

SEED Subsystems

Subsystem	#Specific
Arginine and Ornithine Degradation	2
Polyamine Metabolism	2
ATP-dependent RNA helicases, bacterial	1
Acid resistance mechanisms	1
Auxin degradation	1
Biogenesis of cytochrome c oxidases	1
CBSS-262719.3.peg.410	1
Glycine and Serine Utilization	1
Lipid A modifications	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
Orphan regulatory proteins	1
Oxidative stress	1
Peptidoglycan Biosynthesis	1
Photorespiration (oxidative C2 cycle)	1
Pyruvate Alanine Serine Interconversions	1
Transport of Iron	1
Type IV pilus	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Peptidoglycan biosynthesis
• Fructose and mannose metabolism
• Urea cycle and metabolism of amino groups
• Glycine, serine and threonine metabolism
• Arginine and proline metabolism
• Tryptophan metabolism
• Lipopolysaccharide biosynthesis
• Methane metabolism
• Purine metabolism
• Pyrimidine metabolism
• Cysteine metabolism
• Lysine degradation
• Phenylalanine metabolism
• Benzoxazinone biosynthesis
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Nucleotide sugars metabolism
• Keratan sulfate biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Biotin metabolism
• Folate biosynthesis
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Biosynthesis of siderophore group nonribosomal peptides
• Biosynthesis of phenylpropanoids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-serine degradation	3	3	3
betanidin degradation	1	1	1
arginine dependent acid resistance	1	1	1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	3	2
D-serine degradation	3	3	2
putrescine biosynthesis II	3	3	2
L-cysteine degradation II	3	2	2
L-tryptophan degradation II (via pyruvate)	3	2	2
GDP-mannose biosynthesis	4	4	2
superoxide radicals degradation	2	2	1
pseudouridine degradation	2	1	1
D-mannose degradation II	2	1	1
putrescine biosynthesis I	2	1	1
baicalein degradation (hydrogen peroxide detoxification)	2	1	1
methanol oxidation to formaldehyde IV	2	1	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	1	1
D-mannose degradation I	2	1	1
glycine betaine degradation III	7	7	3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
glycine betaine degradation I	8	6	3
glycine degradation	3	3	1
ethanol degradation IV	3	3	1
L-methionine biosynthesis II	6	5	2
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
mannitol biosynthesis	3	1	1
reactive oxygen species degradation	4	4	1
superpathway of polyamine biosynthesis II	8	7	2
superpathway of putrescine biosynthesis	4	3	1
L-mimosine degradation	8	4	2
mannitol degradation II	4	2	1
spermidine biosynthesis III	4	2	1
glutathione-mediated detoxification I	8	3	2
luteolin triglucuronide degradation	4	1	1
xanthommatin biosynthesis	4	1	1
1,5-anhydrofructose degradation	5	2	1
colanic acid building blocks biosynthesis	11	9	2
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	2
β-(1,4)-mannan degradation	7	2	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
superpathway of polyamine biosynthesis I	8	7	1
purine nucleobases degradation II (anaerobic)	24	16	3
sesamin biosynthesis	8	1	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
peptidoglycan recycling II	10	8	1
justicidin B biosynthesis	10	1	1
matairesinol biosynthesis	10	1	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	5	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	1
superpathway of L-arginine and L-ornithine degradation	13	7	1
peptidoglycan recycling I	14	10	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
superpathway of arginine and polyamine biosynthesis	17	16	1