Experiment set1IT021 for Enterobacter asburiae PDN3

Plant=poplar; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=quartz_sand; Collection=outgrowth_in_MGL; Time=7_days

Group: in planta
Media: MS_media + Plant=poplar; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=quartz_sand; Collection=outgrowth_in_MGL; Time=7_days
Culturing: EnterPDN3_ML4
By: Doty lab on 5/1/20
Media components: 1650 mg/L Ammonium Nitrate, 6.2 mg/L Boric Acid, 332.2 mg/L Calcium chloride, 0.025 mg/L Cobalt chloride hexahydrate, 0.025 mg/L Copper (II) sulfate pentahydrate, 37.26 mg/L EDTA (disodium salt), 27.8 mg/L Iron (II) sulfate heptahydrate, 2 mg/L Glycine, 180.7 mg/L Magnesium sulfate, 16.9 mg/L Manganese sulfate, 100 mg/L m-Inositol, 0.5 mg/L Nicotinic Acid, 0.83 mg/L Potassium iodide, 1900 mg/L Potassium nitrate, 170 mg/L Potassium phosphate monobasic, 0.5 mg/L Pyridoxine HCl, 0.25 mg/L Sodium Molybdate Dihydrate, 0.1 mg/L Thiamine HCl, 8.6 mg/L Zinc sulfate heptahydrate, 20 g/L Sucrose

Specific Phenotypes

For 1 genes in this experiment

For in planta Plant=poplar; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=quartz_sand; Collection=outgrowth_in_MGL; Time=7_days in Enterobacter asburiae PDN3

For in planta Plant=poplar; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=quartz_sand; Collection=outgrowth_in_MGL; Time=7_days across organisms

SEED Subsystems

Subsystem	#Specific
Acetyl-CoA fermentation to Butyrate	1
Anaerobic respiratory reductases	1
Butanol Biosynthesis	1
Isobutyryl-CoA to Propionyl-CoA Module	1
Isoleucine degradation	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid metabolism
• Valine, leucine and isoleucine degradation
• Geraniol degradation
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Brassinosteroid biosynthesis
• Caprolactam degradation
• Biosynthesis of terpenoids and steroids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyruvate fermentation to butanol II (engineered)	6	4	1
fatty acid β-oxidation I (generic)	7	6	1
pyruvate fermentation to hexanol (engineered)	11	8	1
oleate β-oxidation	35	32	3
1-butanol autotrophic biosynthesis (engineered)	27	19	1