Experiment set1IT019 for Pontibacter actiniarum KMM 6156, DSM 19842
marine broth with Nickel (II) chloride hexahydrate 2 mM
Group: stressMedia: marine_broth_2216 + Nickel (II) chloride hexahydrate (2 mM)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 3.9 generations
By: Adam on 29-Mar-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1731 A3
Specific Phenotypes
For 11 genes in this experiment
For stress Nickel (II) chloride hexahydrate in Pontibacter actiniarum KMM 6156, DSM 19842
For stress Nickel (II) chloride hexahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Flavanone biosynthesis | 1 |
| Thiamin biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid metabolism
- Purine metabolism
- Pyrimidine metabolism
- Lysine degradation
- Tryptophan metabolism
- Benzoate degradation via CoA ligation
- Nicotinate and nicotinamide metabolism
- Flavonoid biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| aromatic polyketides biosynthesis | 2 | 1 | 1 |
| NAD salvage pathway III (to nicotinamide riboside) | 3 | 2 | 1 |
| phloridzin biosynthesis | 3 | 1 | 1 |
| UTP and CTP dephosphorylation I | 7 | 5 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 10 | 3 |
| inosine 5'-phosphate degradation | 4 | 4 | 1 |
| guanosine nucleotides degradation III | 4 | 4 | 1 |
| guanosine nucleotides degradation II | 4 | 3 | 1 |
| adenosine nucleotides degradation I | 8 | 5 | 2 |
| purine nucleotides degradation I (plants) | 12 | 7 | 3 |
| guanosine nucleotides degradation I | 4 | 2 | 1 |
| xanthohumol biosynthesis | 4 | 1 | 1 |
| naringenin biosynthesis (engineered) | 4 | 1 | 1 |
| adenosine nucleotides degradation II | 5 | 4 | 1 |
| flavonoid biosynthesis | 5 | 1 | 1 |
| superpathway of guanosine nucleotides degradation (plants) | 6 | 3 | 1 |
| superpathway of purines degradation in plants | 18 | 8 | 3 |
| ureide biosynthesis | 7 | 5 | 1 |
| flavonoid di-C-glucosylation | 15 | 4 | 2 |
| tunicamycin biosynthesis | 9 | 2 | 1 |
| flavonoid biosynthesis (in equisetum) | 10 | 1 | 1 |
| NAD salvage (plants) | 11 | 6 | 1 |