Experiment set1IT018 for Pontibacter actiniarum KMM 6156, DSM 19842

Compare to:

marine broth with Nickel (II) chloride hexahydrate 1 mM

Group: stress
Media: marine_broth_2216 + Nickel (II) chloride hexahydrate (1 mM)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.7 generations
By: Adam on 29-Mar-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1731 A2

Specific Phenotypes

For 33 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Pontibacter actiniarum KMM 6156, DSM 19842

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Cobalt-zinc-cadmium resistance 2
Copper homeostasis: copper tolerance 2
De Novo Purine Biosynthesis 2
Phosphate metabolism 2
Flavanone biosynthesis 1
Oxidative stress 1
Potassium homeostasis 1
Streptococcal Mga Regulon 1
Transport of Zinc 1
Tryptophan synthesis 1
Two-component regulatory systems in Campylobacter 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate 2 2 1
aromatic polyketides biosynthesis 2 1 1
5-aminoimidazole ribonucleotide biosynthesis I 5 5 2
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 5 2
phloridzin biosynthesis 3 1 1
naringenin biosynthesis (engineered) 4 1 1
xanthohumol biosynthesis 4 1 1
5-aminoimidazole ribonucleotide biosynthesis II 5 4 1
flavonoid biosynthesis 5 1 1
L-tryptophan biosynthesis 6 6 1
flavonoid di-C-glucosylation 15 4 2
flavonoid biosynthesis (in equisetum) 10 1 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 2
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
superpathway of L-tryptophan biosynthesis 13 13 1
superpathway of aromatic amino acid biosynthesis 18 17 1
superpathway of purine nucleotides de novo biosynthesis II 26 23 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 1
superpathway of chorismate metabolism 59 39 1