Experiment set1IT018 for Escherichia coli BW25113

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L-Fucose carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + L-Fucose (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 4.9 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 11 genes in this experiment

For carbon source L-Fucose in Escherichia coli BW25113

For carbon source L-Fucose across organisms

SEED Subsystems

Subsystem #Specific
L-fucose utilization 6
L-rhamnose utilization 2
Methylglyoxal Metabolism 2
Alanine biosynthesis 1
Branched-Chain Amino Acid Biosynthesis 1
Calvin-Benson cycle 1
L-fucose utilization temp 1
Pyruvate Alanine Serine Interconversions 1
Transport of Manganese 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-arabinose degradation II 4 4 4
ethylene glycol degradation 2 2 2
L-lactaldehyde degradation (anaerobic) 1 1 1
L-fucose degradation I 4 4 3
D-arabinose degradation I 2 2 1
L-alanine biosynthesis I 2 2 1
L-lactaldehyde degradation (aerobic) 2 2 1
superpathway of fucose and rhamnose degradation 12 12 5
lactate biosynthesis (archaea) 5 3 2
methylglyoxal degradation IV 3 2 1
methylglyoxal degradation V 3 2 1
nucleoside and nucleotide degradation (halobacteria) 6 3 2
superpathway of glycol metabolism and degradation 7 7 2
superpathway of L-alanine biosynthesis 4 4 1
methylglyoxal degradation VI 4 1 1
superpathway of methylglyoxal degradation 8 6 1
L-rhamnose degradation II 8 4 1
Rubisco shunt 10 9 1
superpathway of pentose and pentitol degradation 42 18 4
Calvin-Benson-Bassham cycle 13 10 1
oxygenic photosynthesis 17 11 1
ethene biosynthesis V (engineered) 25 18 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 1
1-butanol autotrophic biosynthesis (engineered) 27 19 1