Experiment set1IT013 for Escherichia coli BW25113

D-Galactose carbon source

Group: carbon source
Media: M9 minimal media_noCarbon + D-Galactose (20 mM)
Culturing: Keio_ML9, tube, Aerobic, at 37 (C), shaken=200 rpm
Growth: about 5.1 generations
By: Kelly on 6/19/2014
Media components: 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 5 genes in this experiment

For carbon source D-Galactose in Escherichia coli BW25113

For carbon source D-Galactose across organisms

SEED Subsystems

Subsystem	#Specific
Lactose and Galactose Uptake and Utilization	4
L-Arabinose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Nucleotide sugars metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-galactose detoxification	3	3	2
stachyose degradation	7	7	3
D-galactose degradation I (Leloir pathway)	5	5	2
1,3-propanediol biosynthesis (engineered)	9	7	1